中医不同治法对绝经后骨质疏松症大鼠骨骼、骨骼肌SDF-1含量影响的比较研究

目的探究用中医滋补肾阴、温补肾阳、健脾、补肾健脾治法治疗绝经后骨质疏松症是否会引起骨骼、骨骼肌中SDF-1水平的变化,从而扩展中医防治骨质疏松症的现代医学机制。方法除正常组外,将去卵巢致骨质疏松症的大鼠随机分为模型组、补肾阴组、补肾阳组、健脾组、补肾健脾组和福善美组。采用酶联免疫吸附法检测各组大鼠血清ALP、TRACP及骨骼、骨骼肌中SDF-1的水平。结果模型组大鼠血清ALP和TRACP水平均比正常组显著升高(P<0.01);各治疗组均比模型组显著降低(P<0.01),以福善美组降低最为明显,其次为补肾健脾组。②模型组大鼠的骨骼和骨骼肌SDF-1水平均比正常组显著升高(P<0.01);与模型组相比,补肾阳组、福善美组大鼠骨组织SDF-1水平显著降低(P<0.01),健脾组、补肾阴组也降低(P<0.05),福善美组、健脾组大鼠的骨骼肌SDF-1水平显著降低(P<0.01)。结论骨质疏松症的发生与骨骼、骨骼肌中SDF-1水平的升高有关。②补肾阴、补肾阳、健脾和补肾健脾法可以减少绝经后骨质疏松症大鼠骨骼、骨骼肌中SDF-1的水平,从而对骨质疏松症起到一定的防治作用。

Comparative study on the effect of different TCM treatment on SDF-1 levels of the bone and skeletal muscle in postmenopausal osteoporotic mice

Objective To observe the change of SDF-1 levels in the bone and skeletal muscle during the treatment of postmenopausal osteoporosis with TCM nourishing the kidney-yin, warming the kidney-yang, tonifying spleen, and tonifying kidney and spleen, and to explore the modern medical mechanism of TCM in the prevention and treatment of osteoporosis. Methods Except the normal group, ovariectomized rats with osteoporosis were randomly divided into model group, tonifying kidney-yin group, tonifying kidney-yang group, invigorating spleen group, tonifying kidney and invigorating spleen group, and Fosamax group. Serum ALP, TRACP, bone and skeletal muscle SDF-1 levels in rats of each group were examined with ELISA method. Results 1) Compared to the normal group, ALP and TRACP levels in the model group increased significantly (P<0.01). All treated-groups showed decreased levels of ALP and TRACP comparing to those in the model group (P<0.01), especially in the Fosamax group, followed by that in the tonifying kidney and invigorating spleen group. 2) SDF-1 levels in the bone and skeletal muscle in the model group were significantly higher than those in the normal group (P<0.01). Compared to the model group, bone SDF-1 levels in the tonifying kidney-yang group and the Fosamax group decreased significantly (P<0.01), followed by that in the invigorating spleen group and tonifying kidney-yin group (P<0.05). Skeletal muscle SDF-1 levels in the Fosamax group and invigorating spleen group were significantly lower than those in the model group (P<0.01). Conclusion 1) The occurrence of osteoporosis is related to the increase of SDF-1 expression in the bone and skeletal muscle. 2) Treatment with nourishing the kidney-yin, warming the kidney-yang, invigorating spleen and tonifying kidney and spleen show effects on postmenopausal osteoporosis by down-regulating SDF-1 expressions in the bone and skeletal muscle.