百里香精油的化学成分分析及其抗菌活性

目的：分析百里香精油的化学成分及其抗菌活性。方法：采用气相色谱一质谱联用技术（GC—MS）鉴定百里香精油的化学成分，并采用琼脂扩散法考察其对大肠杆菌及金黄色葡萄球菌的抑菌直径。结果：百里香精油共分离出23个峰，鉴定出其中9种化学成分，主要是百里香酚（44．36％）和对伞花烃（31．04％）；百里香精油对大肠杆菌和金黄色葡萄球菌的抑菌直径分别是32mm±0．5mm和40．5mm±1．0mm。结论：百里香精油主要成分是百里香酚和对伞花烃，其对大肠杆菌和金黄色葡萄球菌具有较强的抗菌活性。

Analysis of Chemical Composition of Thymus Essential Oil and its Antibacterial Activity

Objective： To analyses the chemical composition of thymus essential oil and its antibacterial acitivity. Methods：The components of essential oil were identified by GC- MS,and the diameters of inhibition zones of it against Escherichia coli and Staphylococcus aureus were investigated by agar disc diffusion method. Results：23 components of thymus essential oil were separated and 9 of them were identified,the main constituents were thymol （44. 36% ） and p - cymene （31.04%） ;the diameters of inhibition zones of essential oil towards Escherichia eoli and Staphylococcus aureus were 32mm ± 0. 5mm and 40. 5mm± 1.0mm. Conclusion：The major components of thymus essential oil were thymol and p - cymene, and it exhibited higher antibacterial activity against Escherichia coli and Staphylococcus aureus.