转移性人小细胞肺癌裸鼠模型的建立

目的建立转移性人小细胞肺癌(SCLC)的裸鼠模型,探讨其用于人SCLC的治疗研究的可行性。方法 RPMI 1640培养基培养的人SCLC细胞株NCI-H446用无血清培养液重悬成1×107个/mL,取0.1 mL细胞悬液接种于BALB/c-nu裸鼠胫骨结节骨髓腔内,构建裸鼠模型并观察腿部肿瘤生长情况及肺等器官的转移情况。免疫组化染色检测小细胞肺癌标志物CD56及神经上皮干细胞标志物netsin的表达情况。结果经胫骨结节骨髓腔注射的方法注射细胞后裸鼠腿部均形成原位瘤,肺部转移6/6只,肝、脾等脏器未见转移灶。肿瘤组织细胞CD56阳性率>75%,nestin阳性率为4%～12%。结论经胫骨结节骨髓腔注射的方法可以形成弥散性肺部肿瘤,其他各器官则未见肿瘤块,可以为人SCLC实验性治疗的研究提供一种高效的实验模型。

Establishment of nude mouse model with metastatic human small cell lung cancer

Objective To establish a nude mouse model with metastatic human small cell lung cancer(SCLC) and explore the feasibility of the model used to study the treatment of human SCLC.Methods Human SCLC cell line NCI-H446 was cultured in RPMI 1640 media and resuspended with free-serum media to prepare cell suspension with final concentration of 1×107 cells/mL.The mouse model was established by injecting 0.1 mL of the cell suspension into the right proximal tibia of BALB/c-nu nude mouse and the growth and metastasis of the tumor in the nude mouse were observed.The expressions of both CD56,the specific marker of small cell lung cancer,and nestin,the marker of neuroepithelial stem cells,were detected by immunohistochemistry staining.Results Orthotopic tumors in hind legs were produced successfully in all the nude mouse after NCI-H446 cells were injected,and the incidence of lung metastasis was 100%(6/6).Nevertheless,the metastasis of other organs e.g.,liver,spleen,etc.was not found.The positive rates for CD56 and nestin in tumor cells were more than 75% and from 4% to 12%,respectively.Conclusion The nude mouse model with metastatic human SCLC was established successfully by injecting lung cancer NCI-H446 cells into the tibia bone marrow of nude mouse,which provided an animal model for studying the treatment of human SCLC.