大鼠移植性BERH-2肝癌染色质——蛋白质的免疫化学

用大鼠移植性BERH-2肝癌染色质蛋白质免疫家兔,以酶联免疫吸附测定法(ELISA)定量测定其免疫反应,表明抗肝癌血清和肝癌染色质蛋白比正常肝染色质蛋白免疫反应强。用酶联免疫转移电泳印迹法(EITB)检测,指示肝癌染色质蛋白中存在着正常肝所没有的蛋白条带;正常肝染色质蛋白中的某些蛋白条带却在肝癌中不出现或含量明显减少,结果表明肝癌与正常肝染色质蛋白质存在着质和量的差别,这些免疫特异的蛋白质可能与肝癌变有关。

IMMUNOCHEMISTRY ON CHROMOSOMAL PROTEINS PROM RAT TRANSPLANTABLE BERH-2 HEPATOMA

Antisera raised against rat transplantable BEEH-2 hepatoma chromosomal protein were used for immunochemical method of ELISA and enzyme-linked immunotransfer blot (EITB). We observed differences in the antigenicity of chromosomal proteins from transplantable BEEH-2 hepatoma and normal liver. By ELISA, the immunoreaotivity of the hepatoma chromosomal proteins is much stronger than that of normal liver, when both were assayed with preabsorbed antisera at a 1/3 200 dilution. EITB showed the presence of three protein bands with molecular weights of 29k, 36k, and 90 k daltons respectively in rat hepatoma chromosomal proteins but not in the normal rat liver, and three protein bands with molecular weights of 33k, 55k, 65k, daltons respectively in normal rat liver but not in hepatoma chromosomal proteiaos.Besides, the quantities of proteins with molecular weights of 24k, and 43k, daltons in normal rat liver; were higher than those from hepatoma chromatin. We suggest that this diversity of chromosomal proteins may be related to the malignant. state and the chromosomal nonhistone protein may be a protein marker for cell differentiation and neoplasia.