四逆散有效组分改善睡眠作用的量效、时效关系及作用机制研究

目的:在研究四逆散有效组分改善睡眠作用的量效、时效关系的基础上从NO信号通路探讨四逆散有效组分改善睡眠的作用机制。方法:采用戊巴比妥钠所致小鼠睡眠实验方法,以小鼠睡眠时间为指标研究四逆散有效组分改善睡眠作用的量效、时效关系,四逆散有效组分联合L-精氨酸、N-硝基-L-精氨酸甲酯应用改善睡眠作用,并测定给予四逆散有效组分后小鼠脑干与丘脑的NO含量。结果:四逆散有效组分改善睡眠作用时效关系明显,最短起效时间为连续给药4天,量效关系复杂在给药剂量增加到660.00mg/kg时达到药效高峰,在给药剂量1320.00mg/kg时药效减弱;给予四逆散有效组分后小鼠脑干与丘脑的NO含量增加;联合L-精氨酸给药后,睡眠持续时间相对于四逆散有效组分有延长作用;联合N-硝基-L-精氨酸甲酯给药后,睡眠持续时间相对于四逆散有效组分有缩短作用。结论:四逆散有效组分改善睡眠作用时效关系明显、量效关系复杂,其改善睡眠的作用NO信号通路参与了介导。

Research on the Role of SNSC on Improving Sleep Based on NO Signaling Pathway

Objective:The study researched the mechanisms of improving sleep by SNSC through NO signaling pathway based on dose-response and time-concentration.Method:The study researched the dose-response and time-concentration of the effect of SNSC determined with sleep period adopt with sodium pentobarbital collaborative experiment.The effect of improved-sleeping of SNSC combined with L-Arg or L-NAME.The NO content of hypothalamus and brain stem in mice given SNSC was detected.Results:The effect of improved-sleeping of SNSC time-concentration was obvious and the shortest time of taking effect was the 4th day of continuous administration.The dose-response was complex that the drug effect reached the top of the peak after the dose adding to 660.00 mg/kg and the drug effect weakened after the dose adding to 1320.00 mg/kg.The content of NO of brain stem and medial thalamus of mice increased after administration with SNSC.After combined with L-Arg,the sodium pentobarbital-induced sleep time prolonged compared to SNSC in mice.SNSC combined with L-NAME can shorten sodium pentobarbital-induced sleep time compared to SNSC in mice.Conclusion:SNSC improved sleep based on NO signaling pathway with obvious time-concentration and complex dose-response.