吴茱萸碱对涎腺腺样囊性癌SACC-M细胞生长的调控影响

目的:研究茱萸碱对涎腺腺样囊性癌SACC-M细胞生长的调控影响,为临床治疗涎腺腺样囊性癌提供理论依据。方法:选择口腔颌面外科手术治疗的20例涎腺腺样囊性癌患者的癌组织细胞ACC-M,置于含有RPMI1640、小牛血清、青霉素及链霉素的培养基中,恒温37℃下传代培养。再使用MTT比色实验将对数生长期的细胞悬浮液种于孔板内培养24h后,按浓度由小到大5种不同浓度比例加入吴茱萸碱溶液,另外设1组空白对照,1组调零组,进行培养后分别加入MTT、二甲基亚砜溶液用酶标法测定各组吸光度值并计算细胞生长的抑制率,显微镜下观察细胞生长状况,用流式细胞仪检测细胞凋亡状况。结果:MTT比色试验结果显示,吴茱萸碱对SACC-M细胞的生长具有明显的抑制效果,它的抑制率随时间的延长而增长,在药物作用后的24、48、72h的抑制率分别为35%,65%,85%,经过对细胞的抑制作用与药物浓度做二变量相关性分析可知二者呈正相关性(r=1.194,r0.01)。显微镜下观察可见对照组细胞生长迅速,实验组生长缓慢;流式细胞仪下可见实验组大量细胞死亡,对照组凋亡率低。结论:吴茱萸碱对涎腺腺样囊性癌细胞具有生长抑制作用,它可以控制SACC-M细胞的增殖与凋亡,且具有随浓度升高药效增强的趋势,可以将此运用于SACC的治疗。

Regulatory Impact of Evodiamine on Salivary Adenoid Cystic Carcinoma SACC-M Cell Growth

Objective:To study the effect of evodiamine on salivary adenoid cystic carcinoma SACC-M cell growth regulation and provide the theoretical basis for salivary adenoid cystic carcinoma.Methods:20 cases of adenoid cystic carcinoma patients with cancer cell of ACC-M were selected.They were placed in substrate containing RPMI1640,calf serum,penicillin and streptomycin in constant temperature 37 ℃.The MTT colorimetric experiment was used to grow the the cell suspension,which phase of cells is exponential,in well plate for 24h.Evodiamine solution was added into the solution with the concentration from small to large,another group was set up as control,the last group as zero group,cultured and then they were added MTT,DMSO in each group and determined by enzyme linked immunosorbent assay absorbance values and also calculated the inhibition rate of cell growth.Cell growth was observed under the microscope,and cell apoptosis was detected by flow cytometry conditions.Results:After MTT colorimetric test,the results showed that evodiamine has obvious inhibiting effect on SACC-M cell growth,and its inhibition rate increased with the extension of time and after the 24h,48h,72h inhibition rates were 35%,65%,85%.Two had positive correlation after the cell inhibition and drug concentration in two variables correlation analysis(r=1.194,P<0.01).Microscopically controls cell growth was rapid,the experimental group grow slowly.Flow cytometry can observe massive cell death in experimental group,but control group with low death rate.Conclusion:Evodiamine adenoid cystic carcinoma cell growth inhibition can control the proliferation and apoptosis of SACC-M cells,with efficacy increasing trend with the concentration used in the treatment of SACC.