| 高效液相色谱法测定锦灯笼药材中维生素C含量 |
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| 引用本文： | 徐保利,管慧洁,王冰.高效液相色谱法测定锦灯笼药材中维生素C含量[J].中华中医药学刊,2012(4):877-878. |
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| 作者姓名： | 徐保利 管慧洁 王冰 |
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| 作者单位： | 辽宁中医药大学 |
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| 摘 要： | 目的:建立一种测定锦灯笼药材中维生素C含量的方法。方法:色谱柱采用Agilent TC-18(4.6mm×150mm,5μm),柱温为25℃,以0.1%草酸水为流动相,流速为0.5 mL.min-1,UV检测器,检测波长为265nm。结果:样品进样量在4.72～23.6μg时呈良好的线性关系(r=0.9997);加样回收率分别为99%和97%,RSD为2.52%和2.12%。结论:本法操作简便,分离效果好。
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| 关 键 词： | HPLC 锦灯笼 维生素C |
HPLC Determination of Vitamin C of Physalis Calyx Seu Fructus |
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| XU Bao-li,GUAN Hui-jie,WANG Bing.HPLC Determination of Vitamin C of Physalis Calyx Seu Fructus[J].Chinese Archives of Traditional Chinese Medicine,2012(4):877-878. |
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| Authors: | XU Bao-li GUAN Hui-jie WANG Bing |
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| Institution: | (Liaoning University of Traditional Chinese Medicine,Dalian116600,Liaoning,China) |
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| Abstract: | Objective:To establish a HPLC method for determining Vitamin C of Physalis Calyx Seu Fructus.Method:HPLC analysis was performed on a Agilent TC-18 analytical column(150mm×4.6mm,5μm)using 0.1% aqueous oxalic acid as the mobile phase with UV absorption 265 nm.The flow rate was 0.5mL·min-1.Results:The calibration curves were linear for Vitamin C within 4.72~23.6μg(r=0.9997).The recoveries were 99% and 97%(RSD=2.52% and 2.12%).Conclusion:This method was simple and the results were satisfactory. |
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| Keywords: | HPLC Physalis Calyx Seu Fructus Vitamin C |
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