六味地黄丸对2型糖尿病大鼠血清RBP4及血糖血脂的影响

目的:观察六味地黄丸对2型糖尿病模型大鼠血清RBP4及血糖血脂的影响。方法:36只健康雄性wistar大鼠随机分为4组:空白对照组(组A)、模型对照组(组B)、六味地黄丸组(组C)、吡格列酮组(组D)。高脂饲料配合小剂量链脲佐菌素(STZ)30mg/kg建立2型糖尿病大鼠模型。空白对照组与模型对照组灌胃生理盐水,其余各组灌胃给予相应药物,分别造模前、造模后、给药第2、5、8周眼眶取血,动态观察大鼠体重、空腹血糖(FPG)、血清胰岛素水平(FINS)、胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)及视黄醇结合蛋白4(RBP4)等指标变化,并计算胰岛素敏感指数(ISI)、胰岛素抵抗指数(HOMA-IR)。结果:8周时,六味地黄丸组(组C)和吡格列酮组(组D)血清RBP4水平、FPG、TC、TG、LDL-C、Fins、HOMA-IR均低于模型组(组B)(P0.05)而体重、HDL-C、ISI则明显高于模型组(组B)(P0.05);在体重方面,六味地黄丸组(组C)和吡咯列酮组(组D)在第2周、第5周均不能增加2型糖尿病大鼠体重(P0.05),在血清RBP4方面,六味地黄丸组(组C)在第2周血清RBP4与模型组相比无明显差异(P0.05),从第5周开始则显示降低(P0.05),吡格列酮组从第2周开始已经低于模型组(P0.05)结论:长期服用六味地黄丸能够改善2型糖尿病大鼠的体重、血糖、血脂水平,改善胰岛素抵抗,降低血清RBP4水平。

Effects of Liuwei Dihuang Pill on RBP4 in Serum,Blood Glucose and Blood Lipid in Type 2 Diabetic Rats

Objective:To study the effect of Liuwei Dihuang Pill on serum RBP4,blood lipid and blood glucose in type 2 diabetic rats.Method:36 healthy male Wistar rats were randomly divided into the nomal group,the model group,group treated with Liuwei Dihuang Pill(Group C)and group treated with Pioglitazone(Group D).Method:With high fat forage diet for five weeks,rats from B,C,and D were injected small dose of streptozotocin(STZ)according to 30mg/kg to establish rats model with type 2 diabetes.Group A and Group B were administrated with normal saline.Group C was administrated with Liuwei Dihuang Pill.Group D was administrated with Pioglitazone.Orbital blood was collected to measure indicators before the model,after the model and 2,5,8 weeks after the treatment.The indicators included weight,fasting plasma glucose(FPG),fasting insulin levels(FINS),cholesterol(TC),triglycerides(TG),high-density lipoprotein(HDL-C),low density lipoprotein(LDL-C)and retinol binding protein 4(RBP4),then calculate insulin sensitivity index(ISI)and insulin resistance index(HOMA-IR).Result:8 weeks after the treatment,the levels of RBP4,FPG,TC,TG,LDL-C,Fins,HOMA-IR of group C and group D were lower than those of group B(P<0.05);and the levels of weight,HDL-C and ISI of group C and group D were higher than those of group B(P<0.05).According to weight,there were no significant difference among group B,C and D(P>0.05)after 2 or 5 weeks of treatment.According to RBP4,there was no significant difference between group B and C after two weeks of treatment(P>0.05).After 5 weeks of treatment,the level of RBP4 of group C was lower than that of group B(P<0.05);there was significant difference between group B and D after both 2 and 5 weeks(P<0.05).Conclusion:Long-term use of Liuwei Dihuang Pill can improve weight of type 2 diabetic rats,reduce blood glucose,blood lipid,insulin resistance and lower the level of blood RBP4.