中药复方益糖康对糖尿病大鼠NaV1.8蛋白及mRNA表达的影响

目的:探讨中药复方益糖康对链脲佐菌素(STZ)致糖尿病模型大鼠背根神经节(DRG)中NaV1.8蛋白及mRNA表达的影响。方法:将健康雄性Wistar大鼠40只,体重200-250g,随机分为空白对照组、模型组和益糖康组,其中,模型组和益糖康组采用STZ 55mg/kg腹腔注射复制糖尿病大鼠模型,然后分别给予安慰剂和益糖康5mL.次-1.天-1灌胃,6周后,采集大鼠背根神经节(DRG)标本,用免疫组化和RT-PCR方法分别检测其NaV1.8蛋白和mRNA表达水平。结果:模型组和益糖康组大鼠DRG中NaV1.8蛋白表达灰度值分别为102.19±10.62,134.04±6.56,mRNA表达积分吸光度比值分别为0.219±0.031,0.137±0.014。模型组大鼠DRG中NaV1.8蛋白和mRNA表达水平均显著上调,益糖康组较模型组NaV1.8蛋白和mRNA表达水平均显著降低。结论:中药复方益糖康可能对NaV1.8通道有阻断作用,是其改善糖尿病痛性神经病症状的机制之一。

Effect of Chinese Herbal Compound Yitangkang on NaV1.8 Protein and mRNA Expressions of Diabetic Rats

Objective:To explore the effect of Chinese herbal compound Yitangkang on NaV1.8 protein and mRNA expressions of diabetic rats.Methods:40 health male Wistar rats,weighted 200~250g,were randomly divided into blank controlled group,model group and Yitangkang group.Model group and Yitangkang group used STZ 55mg/kg ip to copy diabetes rats model,and were respectively given comfort agent and Yitangkang 5mL/d ig for 6 weeks,rats DRG specimens were collected,with immunohistochemistry and RT-PCR methods were used respectively to detect its NaV1.8 protein and mRNA expression levels.Results:Gray value of NaV1.8 protein expression in model group and Yitangkang group were respectively 102.19±10.62,134.04±6.56,integral optical density ratio of NaV1.8 mRNA expression were respectively 0.219±0.031,0.137±0.014.Results showed that for model group,NaV1.8 protein and mRNA expression in rat DRG increased markedly,compared with the model group,NaV1.8 protein and mRNA expression levels in Yitangkang group were significantly reduced.Conclusion:Chinese herbal compound Yitangkang may be blocking for the NaV1.8 channel,which is proved to be one of the mechanisms of diabetic painful neuropathy symptoms.