双氢青蒿素抑制人胰腺癌细胞SW-1990的实验研究

目的:研究双氢青蒿素对人胰腺癌细胞株SW-1990增殖及凋亡的影响,并探讨其作用机制。方法:采用细胞增殖/毒性检测试剂盒(Cell Counting Kit-8,CCK-8)检测不同浓度双氢青蒿素(dihydroartemisinin,DHA)对人胰腺癌细胞株SW-1990增殖的影响;流式细胞术Annexin V-FITC和PI双标染色法检测不同浓度DHA对其凋亡的影响;并且用激光共聚焦显微镜观察在Hoechst 33342/PI荧光双染色下胰腺癌细胞SW-1990形态学变化;RT-PCR检测DHA对胰腺癌细胞SW-1990中端粒酶催化亚单位hTERT mRNA表达的影响。结果:DHA在体外可以明显的抑制SW-1990的增殖,并且具有浓度-时间依赖性;DHA在体外能明显诱导SW-1990细胞的凋亡;DHA可以抑制胰腺癌细胞SW-1990中hTERT mRNA的表达,并且与浓度呈正相关。结论:DHA在体外抑制胰人腺癌细胞SW-1990的增殖、诱导其凋亡,其作用机制可能是抑制了肿瘤细胞中端粒酶催化亚单位hTERT mRNA的表达。

Experiment Research on Apoptosis of Human Pancreatic Adenocarcinoma Cell Induced by Dihydroartemisinin

Objective:To investigate the anticancer effect of dihydroartemisinin on human pancreatic cancer cell line SW-1990 in vitro and the possible mechanis-m.Methods:For cultured cells,cell growth was determined by the Cell Counting Kit-8(CCK-8)assay and apoptosis was evaluated by flow cytometry analysis stained with Annexin V-FITC/PI.Pathomorphism changes of SW-1990 cells were observed by laser scanning confocal microscope(LSCM)after administration of DHA through Hoechst 33342/PI fluorescence staining.Besides:RT-PCR was applied to detect the expression of hTERT mRNA.Results:DHA significantly inhibited the proliferation of SW-1990 cells,in a time-concentration dependent manner;and induced their apotosis respectively in vitro.DHA can also inhibit the expression of hTERT mRNA,in a time-dependent manner.Conclusion:DHA could inhibit the proliferation of Human Pancreatic Adenocarcinoma Cell Line SW-1990,and induce their apoptosis in vitro.The mechanism is that DHA can significantly inhibit the expression of hTERT mRNA in SW-1990.