| 滋补脾阴方药对脾阴虚糖尿病大鼠海马内质网应激影响的研究 |
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| 引用本文： | 梁丽娜,常晓慧,战丽彬,施翔,胡守玉,闫云,郑路平,张福良. 滋补脾阴方药对脾阴虚糖尿病大鼠海马内质网应激影响的研究[J]. 中华中医药学刊, 2012, 0(9): 1969-1972 |
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| 作者姓名： | 梁丽娜 常晓慧 战丽彬 施翔 胡守玉 闫云 郑路平 张福良 |
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| 作者单位： | 大连医科大学附属二院中医科;中国人民解放军第210医院中医科;大连医科大学中西医结合研究院;潍坊市中医院老年病科;大连船舶康复医院神经内科;南阳市第一人民医院急诊科;大连医科大学公共卫生学院 |
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| 基金项目： | 国家自然科学基金资助项目(30772847);高等学校博士学科点专项科研基金资助项目(20070161004);辽宁省高校优秀人才支持计划资助项目(LR2001015) |
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| 摘 要： | 目的:探讨滋补脾阴方药(ZiBu PiYin Recipe,ZBPYR)对脾阴虚糖尿病大鼠海马内质网应激相关分子表达的影响。方法:高脂饲料喂养联合小剂量链脲佐菌素(streptozocin,STZ)注射建立2型糖尿病模型,在此基础上,通过复合因素造模的方法建立脾阴虚糖尿病模型,以水迷宫实验评价模型大鼠是否出现学习记忆障碍,之后观察各组大鼠海马内质网应激相关分子葡萄糖调节蛋白78(glucose regulated protein 78,GRP78)、蛋白激酶样内质网激酶(protein kinase RNA-like endoplasmic reticulum kinase,PERK)和真核转录因子2的α亚单位(eukaryotic translation initiation factor 2 subunitα,eIF2α)表达的变化。结果:ZBPYR治疗后GRP78 mRNA水平降低(P0.05),GRP78、磷酸化PERK、磷酸化eif2α蛋白水平明显降低(P0.05),与脾阴虚糖尿病组相比,差异有统计学意义。结论:滋补脾阴方药可能是通过影响内质网应激PERK信号传导改善脾阴虚糖尿病大鼠学习记忆障碍。
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| 关 键 词： | 糖尿病认知下降 滋补脾阴方药 内质网应激 |
Effects of Zibu Piyin Recipe on Endoplasmic Reticulum Stress in Hippocampus of Rats with Diabetes of Spleen-Yin Deficiency |
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| LIANG Li-na,CHANG Xiao-hui,ZHAN Li-bin,SHI Xiang,HU Shou-yu,YAN Yun,ZHENG Lu-ping,ZHANG Fu-liang. Effects of Zibu Piyin Recipe on Endoplasmic Reticulum Stress in Hippocampus of Rats with Diabetes of Spleen-Yin Deficiency[J]. Chinese Archives of Traditional Chinese Medicine, 2012, 0(9): 1969-1972 |
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| Authors: | LIANG Li-na CHANG Xiao-hui ZHAN Li-bin SHI Xiang HU Shou-yu YAN Yun ZHENG Lu-ping ZHANG Fu-liang |
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| Affiliation: | 1.The Second Affiliated Hospital of Dalian Medical University,Dalian 116023,Liaoning,China; 2.No.210 Hospital of PLA,Dalian 116021,Liaoning,China; 3.Institute of Chinese and Western Medicine Affiliated to Dalian Medical University,Dalian 110644,Liaoing,China; 4.Weifang Hospital of Traditional Chinese Medicine,Weifang 261041,Shandong,China; 5.Dalian Shipping Rehabilitation Hospital,Dalian 116013,Liaoning,China; 6.Nanyang First People’s Hospital,Nanyang 362018,Henan,China; 7.Public Health College,Dalian Medical University,Dalian 116044,Liaoning,China) |
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| Abstract: | Objective:To investigate the effects of Zibu Piyin Recipe(ZBPYR)on endoplasmic reticulum stress(ERS)-related protein expression in hippocampus of Spleen-Yin deficiency diabetic rats.Methods:Type 2 diabetes model was established by high fat diet combined with low-dose streptozotocin(STZ)injection.Then we induced spleen-yin deficiency diabetic model by defatigation,hungry-full diet and drenching the drug of damaged yin.To evaluate whether diabetic rats and spleen-yin deficiency diabetic rats were impaired in learning and memory,we performed Morris Water Maze test.We detected the expressions of GRP78,PERK and eIF2,which were related with ERS in cortex of rats.Results:After treating with ZBPYR,the protein levels of GRP78,phosphorylated PERK and phosphorylated eIF2 and the mRNA expression of GRP78 were significantly decreased compared with Spleen-Yin deficiency diabetic rats.Conclusion:ZBPYR may improve learning and memory impairment in Spleen-Yin deficiency diabetic rats by affecting the endoplasmic reticulum stress PERK signaling. |
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| Keywords: | diabetes-associated cognitive decline Zibu Piyin Recipe endoplasmic reticulum stress |
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