三七总皂苷对大鼠腹膜超滤影响的实验研究

目的:观察三七总皂苷对腹膜透析失超滤大鼠模型腹膜形态和超滤功能的影响。方法:实验大鼠随机分为12组:其中高浓度葡萄糖3组(1.5%、2.5%、4.25%);脂多糖(LPS)3组(0.1mg/L、1mg/L、10mg/L);金黄色葡萄糖球菌灭活菌液2组(1011/mL、1015/mL);三七总皂苷干预3组(2.5%葡萄糖溶液+三七总皂苷溶液、1mg/L脂多糖+三七总皂苷溶液、1011/mL金黄色葡萄球菌灭活菌液+三七总皂苷溶液)和正常组。各组大鼠每天腹腔注射相应溶液20mL,20天后做腹膜平衡试验并行腹膜组织病理检查。结果:三七总皂苷可改善高浓度葡萄糖、脂多糖和金黄色葡萄球菌灭菌液引起的腹膜失超滤;高浓度葡萄糖、脂多糖和金黄色葡萄球菌灭菌液均可增加腹膜毛细血管密度,造成毛细血管充血,毛细血管壁增厚,腹膜中炎性细胞浸润。三七总皂苷可以降低腹膜毛细血管密度,明显扩张血管,腹膜毛细血管壁明显增厚、炎性细胞浸润减少。结论:三七总皂苷可改善腹膜超滤功能,其引起的主要腹膜病理改变为腹膜毛细血管密度下降、血管明显扩张,血管壁明显增厚、炎性细胞浸润减少。

Experimental Research on Influence of Panax Pseudo-Ginseng on Peritoneal Morphous and Ultrafiltration Function of Rats

Objective:To observe the influences of Panax pseudo-ginseng on peritoneal morphous and ultrafiltration function in ultrafiltration failure rat models during peritoneal dialysis.Methods:Randomly put the rats into 12 groups,including normal group,three high concentrations of glucose groups(1.5%、2.5%、4.25%),three lipopolysaccharide(LPS) groups(0.1mg/L、1mg/L、10mg/L),two inactivated Staphylococcus aureus groups(1011/mL、1015/mL)and three Panax pseudo-ginseng groups including Panax pseudo-ginseng associated with 2.5% glucose,Panax pseudo-ginseng associated with 1 mg/L LPS and Panax pseudo-ginseng associated with 1011/mL inactivated Staphylococcus aureus.20mL fluid was injected into the rats’ abdominal cavities each day for 20 days.Then make PET and observe the pathological changes in peritoneum.Results:Panax pseudo-ginseng could improve ultrafiltration failure led by high concentration of glucose,LPS and Staphylococcus aureus,high concentration of glucose,LPS and inactivated Staphylococcus aureus could increase peritoneal capillary density,make capillary hyperemia and the walls of capillary thickening and inflammatory cell infiltrate into peritoneal interstitium.Panax pseudo-ginseng could decrease peritoneal capillary density,obviously distend vessels,obviously thicken the walls of capillaries and decrease inflammatory cell infiltrate.Conclusions:Panax pseudo-ginseng could improve ultrafiltration function of peritoneum and Panax pseudo-ginseng could decrease capillary density,distend vessels,thicken the walls of capillaries and decrease inflammatory cell infiltrate.