前列灌肠剂保留灌肠对大鼠的抗炎和小鼠的镇痛作用研究

目的:观察前列灌肠液保留灌肠对大鼠急、慢性炎症的影响和对小鼠的镇痛作用。方法:分别取SD大鼠和昆明种小鼠,随机分成前列灌肠液高、中、低剂量组,阿司匹林组、空白对照(生理盐水)组5组,保留灌肠后观察角叉菜胶注射后引起的大鼠足肿胀度,大鼠腹股沟处植入棉球前后的肉芽肿重量,小鼠注射醋酸30min内出现扭体反应的次数,以及小鼠热板致痛阈值。结果:前列灌肠液高、中、低剂量均有抑制大鼠角叉菜胶足肿胀和大鼠棉球肉芽增生(P0.05),其中给高剂量前列灌肠液时(剂量60mL/kg)有非常明星抑制作用(P0.01),其抑制率与阿司匹林组无显著性差异(P0.05)。前列腺灌肠水煎液低、中、高剂量均能减少小鼠扭体次数,并对热板致小鼠痛阈值均有明显提高作用(P0.05),随剂量增加,镇痛效果越明显,呈现出明显的量效关系。结论:前列腺灌肠剂低、中、高剂量均具有抗菌消炎、镇痛等作用,高剂量时有非常明星的作用.

Anti-Inflammatory and Analgesic Effect of Retention Enema with Qianlie Guanchang Recipe on Experimental Rats

Objective:To explore the anti-inflammatory and analgesic effects of retention enema with Qianlie Guanchang Recipe on experimental rats.Method:SD rats and kunming mice were randomly divided into five groups:low,middle and high dosage groups of Qianlie Guanchang Recipe,aspirin group,and control group.Anti-inflammatory effect of the drug was evaluated in rat models of paw edema induced by carragheenin and models of granuloma by cotton ball.Analgesic activity of Qianlie Guanchang Recipe was evaluated in mice models of pain induced by thermal stimulus and acetic acid.Results:The low,middle and high dosage groups of Qianlie Guanchang Recipe can inhibit paw swelling induced by carragheenin in rats(P<0.05),and reduce the quality of cotton granuloma in rats(P<0.05).Analgesic test showed that the drug can significantily improve the pain threshold of mice in the hot plate test(P<0.05),and effectively inhibit the writhing response frequence induced by acetic acid(P<0.05).The effect of the high dosage(60mL/kg) was the best among the three recipes as compared with the control group(P<0.01).Conclusion:The low,middle and high dosage groups of Qianlie Guanchang Recipe have significant anti-inflammatory and analgesic effects;the effect of the high dosage is the best.