洋金花提取物对肠缺血再灌注大鼠的作用研究

目的:研究洋金花提取物对大鼠缺血再灌注期间肠黏膜的保护作用及机制。方法:采用大鼠肠缺血再灌注模型,大鼠分为假手术组、模型组、阳性对照组、给药组。用Chiu氏评分法观察肠黏膜的损伤程度并检测肠黏膜中肿瘤坏死因子(TNF-α)和白细胞介素(IL-6)。结果:与假手术组比较,模型组可见明显的肠黏膜损伤,TNF-α和IL-6水平明显升高;与模型组比较,给药组肠黏膜病理损伤程度明显减轻,且TNF-α和IL-6水平明显降低。结论:洋金花提取物对大鼠肠缺血再灌注后的肠道具有较好的保护作用,且该作用与TNF-α和IL-6的减少有关。

Research on Effect of Extraction From Flos Daturae on Rats with Intestinal Ischemia-Reperfusion

Objective:To investigate the protective effects and mechanism of extraction from Flos Daturae on rats with ischemia/reperfusion injury.Methods:By using rat model of intesinal I/R injury,Male SD rats were divided into sham-operation group,control group,positive control group and treatment group.Chiu’s count was used to assess the changes in intestinal pathological morphplogy,and the contents of tumor necrosis factorα(TNF-α),interleukin-6(IL-6) in intestinal mucosa were measured.Results:The intestinal mucosa was more clearly in control group compared to those in sham-operation group,and TNF-α and IL-6 contents in control group were significantly increased inversely,the intestinal mucosa was reduced significantly in treatment group compared to those in control group,and TNF-α and IL-6 contents in treatment group were significantly decreased.Conclusion:Pre-treatment with extraction from Flos Daturae has protective effect on intestinal ischemia-reperfusion injury in rats,which may be caused by decreased contents of TNF-α and IL-6.