| HPLC同时测定参附汤中人参皂苷Rg1、Re、Rb1含量的研究 |
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| 引用本文： | 姚静,张春椿,俞冰. HPLC同时测定参附汤中人参皂苷Rg1、Re、Rb1含量的研究[J]. 中华中医药学刊, 2012, 0(4): 788-790 |
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| 作者姓名： | 姚静 张春椿 俞冰 |
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| 作者单位： | 浙江中医药大学 |
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| 基金项目： | 浙江省自然科学基金重大项目(Z2091224) |
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| 摘 要： | 目的:建立参附汤中人参皂苷Rg1、Re、Rb1的含量测定方法。方法:采用Inertsil ODS–SP(4.6mm×250mm,5μm)色谱柱,柱温25℃,流动相为乙腈:水,梯度洗脱,流速:1.0mL.min-1,检测波长203nm。结果:人参皂苷Rg1在12.5～500μg.mL-1线性关系良好,r=0.9999,平均回收率98.86%,RSD为2.32%;人参皂苷Re在12.5～500μg.mL-1线性关系良好,r=0.9999,平均回收率99.09%,RSD为2.22%;人参皂苷Rb1在12.5～500μg.mL-1线性关系良好,r=0.9999,平均回收率99.53%,RSD为1.68%;结论:该方法学考察符合定量要求,结果准确,可用于同时测定参附汤中人参皂苷Rg1,Re,Rb1的含量,为参附汤的质量控制提供依据。
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| 关 键 词： | 参附汤 人参皂苷Rg1 人参皂苷Re 人参皂苷Rb1 HPLC |
Simultaneous Determination of Ginsenoside Rgl,Re,Rb1 in Shenfu Decoction by HPLC |
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| YAO Jing,ZHANG Chun-chun,YU Bing. Simultaneous Determination of Ginsenoside Rgl,Re,Rb1 in Shenfu Decoction by HPLC[J]. Chinese Archives of Traditional Chinese Medicine, 2012, 0(4): 788-790 |
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| Authors: | YAO Jing ZHANG Chun-chun YU Bing |
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| Affiliation: | (College of Pharmaceutical Science,Zhejiang Chinese Medical University,Hangzhou 310053,Zhejiang,China) |
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| Abstract: | Objective: To establish a method for the determination of ginsenoside Rg1,Re,Rb1 in Shenfu Decoction.Method: With a Inertsil ODS SP column(4.6mm 250mm,5μm) at 25℃,the mobile phase consisted of acetonitrile and water for gradient elution with the flow rate at 1.0 mL·min-1.The detection wavelength was 203nm.Result: The resolutions of the determined peaks were fine.The linearity ranges for ginsenoside Rgl,Re,Rbl were 12.5~500μg·mL-1(r=0.9999),12.5~500μg·mL-1(r=0.9999)and 12.5~500μg·mL-1g(r=0.9999),respectively.The average recoveries were 98.86% with RSD 2.32%,99.09% with RSD 2.22%,99.53% with RSD 1.68%.Conclusion: The quantitative method for the determination was feasible and beneficial for the quality control of Shenfu Decoction. |
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| Keywords: | Shenfu Decoction Ginsenoside Rg1 Ginsenoside Re Ginsenoside Rb1 HPLC |
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