| 高效液相色谱法测定左归丸中马钱苷的含量 |
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| 引用本文： | 孙琳林,陈海玲,卢林,刘伦翠,刘艳秋. 高效液相色谱法测定左归丸中马钱苷的含量[J]. 中华中医药学刊, 2012, 0(8): 1792-1793 |
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| 作者姓名： | 孙琳林 陈海玲 卢林 刘伦翠 刘艳秋 |
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| 作者单位： | 牡丹江医学院红旗医院中西医结合科;杭州市中医院老年病科 |
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| 基金项目： | 黑龙江省中医药管理局支撑项目(ZHY10-W70);黑龙江省卫生厅科技计划支撑项目(2010-281);杭州市科技发展计划项目(20100633B22) |
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| 摘 要： | 目的:建立测定左归丸中马钱苷含量的反相高效液相色谱方法。方法:采用Eclipse XDB-C18色谱柱(4.6mm×250mm,5μm);以四氢呋喃-甲醇-乙腈-水(1∶4∶8∶87)为流动相;流速为1.0mL.min-1;柱温为40℃;检测波长236nm。结果:马钱苷进样浓度在0.0079～0.2530mg.mL-1(r=0.9993)范围内呈良好的线性关系,平均回收率(n=6)为101.6%;RSD为2.1%。结论:该方法简便、重现性好,结果准确可靠,可以用于左归丸中马钱苷的含量测定,为左归丸的质量控制提供一定的理论依据。
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| 关 键 词： | 左归丸 马钱苷 高效液相色谱法 含量测定 |
Determination of Loganin in Zuogui Pill by High Performance Liquid Chromatography |
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| SUN Lin-lin,CHEN Hai-ling,LU Lin,LIU Lun-cui,LIU Yan-qiu. Determination of Loganin in Zuogui Pill by High Performance Liquid Chromatography[J]. Chinese Archives of Traditional Chinese Medicine, 2012, 0(8): 1792-1793 |
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| Authors: | SUN Lin-lin CHEN Hai-ling LU Lin LIU Lun-cui LIU Yan-qiu |
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| Affiliation: | 1(1.Department of Combining Chinese with Western Medicine, Hongqi Hospital Affiliated to Mudanjiang Medical College,Mudanjiang 157011,Heilongjiang,China; 2.Department of Geriatrics,Hangzhou Traditional Chinese Medical Hospital,Hangzhou 310007,Zhejiang,China) |
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| Abstract: | Objective:To develop a method for determination of loganin in Zuogui pill by high performance liquid chromatography(HPLC).Methods:A HPLC system was used in the quantitative analysis of hesperidin on the reversed-phase column of Ec1ipse XDB-C18(4.6mm×250mm,5μm)with the mixed solvents of tetrahydrofuran-methanol-acetonitrile-0.05% phksphoric acid water solution.The flow rate was 1.0mL/min.Column temperature was 40℃.The eluate was monitored with UV absorption at 236nm.Results:The linear range of hesperidin was 0.0079 ~ 0.2530mg/mL(r =0.9993).The average recoveries(n=6)were 101.6%,with RSD of 2.1 %.Conclusion:The method is simple,accurate and reliable with good reproducibility.It can be used to evaluate and control the quality of Zuogui pill. |
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| Keywords: | Zuogui pill Loganin HPLC quantitative control |
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