肝纤维化病证结合模型的研究思路

目的:建立适合中医药研究的肝纤维化病证结合动物模型。方法:通过分析近年来肝纤维化中医证候动物摸型研制过程中存在的问题,结合本实验结果对肝纤维化病证结合动物模型的选择、建立方法及评价体系进行探讨分析。结论:以40%CCl4油剂为基础,及(或)其它以中医理论为基础的病因干预因素的综合法复制病证结合肝纤维化模型,肝组织病理上符合肝纤维化疾病模型,大鼠外在一般表现符合中医证的模型,并经中医药反证,表明该病证结合肝纤维化模型复制成功。

Research Thought of Study on Sickness-Syndrome Combination Hepatic Fibrosis Models

Objective:To establish the sickness-syndrome combination hepatic fibrosis models suitable for the research and development work in TCM.Methods:To make use the methods of combining own experimental results and problems of hepatic fibrosis animal model of syndrome for the past few years,study the selection of Chinese medicine syndromes,the methods of construction and standards of evaluation about sickness-syndrome combination hepatic fibrosis models.Conclusion: On the basis of the subcutaneous injection of 40% CCl4,synthetic methods including to etiological factor basing on TCM theory to participate in are used to construct sickness-syndrome combination hepatic fibrosis models.Model rats were consistent with hepatic fibrosis from the pathological changes of hepatic tissue,which manifests that sickness model of hepatic fibrosis is successful.Besides that making use of Chinese raw drugs to treat sickness model is used as counterevidence,Changes of rats general external morphology consistent with syndrome basing on TCM theory,which manifests construction of sickness-syndrome combination models is successful,too.