半夏泻心汤对伊立替康致小细胞肺癌荷瘤鼠胃损伤的改善作用

目的:观察半夏泻心汤对伊立替康(irinotecan,CPT-11)致小细胞肺癌(small-cell lung cancer,SCLC)荷瘤鼠胃损伤是否具有改善作用。方法:建立SCLC荷瘤鼠,将40只荷瘤鼠随机分成4组,高、中、低3个给药组半夏泻心汤剂量分别为20、10、5g.kg-1.d-1,空白组给等量水,灌胃给药,共8天。第3天开始尾静脉注射CPT–11,35 mg.kg-1.d-1,共4天。观察荷瘤鼠食量情况;第8天处死荷瘤鼠,取胃组织,显微镜下观察其病理形态改变,免疫组化测胃黏膜环氧化酶-2(Cyclooxygenase-2,COX-2)表达;另取胃组织,行组织匀浆,离心,酶联免疫吸附法(Enzyme-linked immunosorbent assay,EL ISA)检测胃组织中血栓烷B2(TXB2)和6-酮-前列腺素F1α(6-keta-PGF1α)含量;取舌黏膜组织,显微镜下观察其病理形态改变。结果:半夏泻心汤给药组相较于空白组食量增加,空白组胃黏膜内及黏膜下少量血管有扩张,稍有水肿表现,舌黏膜空白组表皮完整,少量细胞空泡变性,鳞状上皮增生,表面角质层增厚,给药组均好于空白组。胃组织中TXB2和6-keta-PGF1α含量及胃黏膜COX-2表达情况各组无统计学差异。结论:半夏泻心汤对CPT-11致SCLC荷瘤鼠胃损伤具有一定的改善作用。

Study on Improvement of Banxia Xiexin Decoction against Irinotecan-induced Injury of Stomach in Nude Mice Bearing Human Small-cell Lung Cancer

Objective:To observe the improvement of Banxia Xiexin Decoction against irinotecan-induced(CPT-11)injury of stomach in nude mice bearing the tumor of human small-cell lung cancer(SCLC).Methods:We divided it into four groups according the dose of Banxia Xiexin Decoction.The blank group was given the equivalent water,low,middle and high dose groups were given Banxia Xiexin Decoction of 5 g·kg-1·d-1,10g·kg-1·d-1 and 20 g·kg-1·d-1 for 8 days.CPT-11 was given for 35 mg·kg-1·d-1 up to four days from the third day.We recorded food intake,and dealed with mice at the eighth day.We dissected mice for the stomach and tongue tissue and observed the pathological changes of stomach and tongue under microscope.Cyclooxygenase-2(COX-2)of stomach was detected by immunohistochemical methods.TXB2 and 6-keta-PGF1α in stomach were detected by Enzyme-linked immunosorbent assay(ELISA).Results:The appetite examination of the blank group was lower than other three groups.The damage of the mucous membrane in stomach and tongue in the administrated groups was better than the blank group.There were no differences in the expression of COX-2 and concentrations of TXB2 and 6-keta-PGF1α in stomach between the blank group and the administrated groups in statistics.Conclusion:Banxia Xiexin Decoction can improve CPT-11-induced injury of stomach in nude mice bearing human SCLC.