痰热清注射液对内毒素型急性肺损伤大鼠的保护作用

目的:探讨痰热清注射液对内毒素型急性肺损伤大鼠的保护作用及可能的机制。方法:SD大鼠56只,随机分7组:实验对照组;LPS 2h、4h、6h组;痰热清2h、4h、6h组。以尾静脉注射LPS 5mg/kg诱导建立大鼠急性肺损伤模型,以痰热清注射液进行干预,分别于LPS处理后2h、4h、6h取血和肺组织,全自动生化仪检测血清总蛋白(total proteins,TP)含量;测肺湿干比重(wet weight/dry weight,W/D),测定支气管肺泡灌洗液(BALF)中TP含量、肿瘤坏死因子-α(TNF-α)水平及中性粒细胞比例(PMN%),计算肺通透指数(lung permeability index,LPI),显微镜下观察病理。结果:实验中痰热清组大鼠肺组织大体及病理损伤程度均明显轻于LPS组,同时W/D,及BALF中TNF-α水平、中性粒细胞比例,肺通透指数亦较LPS组明显降低。结论:痰热清注射液对内毒素型急性肺损伤大鼠有较好的保护作用,其机制可能与抑制PMN的趋化黏附和调节早期促炎因子相关。

Effects of Tanreqing Injection on Acute Lung Injury Induced by Endotoxin in Rats

Objective:To investigate the effects of Tanreqing injection on acute lung injury induced by endotoxin and to study its probable mechanism.Methods:Fifty-six Sprague-Dawley(SD)rats were randomly divided into seven groups:control group;LPS of 2,4 and 6h groups;Tanreqing of 2,4 and 6h groups.The acute lung injury rat model was induced by injecting LPS(5mg/kg)thought the tail vein injection.At the same time,the rats suffered from acute lung injury were treated with Tanreqing injection.The blood and lung tissue samples were collected at 2,4 and 6h after LPS injection,and the amounts of total proteins(TP)was examined by entire automatic biochemistry mater,the pathological changes of lung were observed,and lung wet/dry weight ratio,protein content,TNF-α,and the ratio of neutrophiles in brochoalveolar lavage fluid(BALF)were calculated.Results:The gross and micrographic injury of lung in Tanreqing groups was milder than those in LPS groups.The W/D ratio,TNF-α contents and the ratio of neutrophiles in BALF and lung permeability index were also markedly reduced in comparison with those in LPS groups.Conclusion:Treatment with Tanreqing injection was effective on protecting rats from ALI induced by intravenous injection with LPS,probably by suppressing pulmonary infiltrates with activated neutrophils and regulating the early proinflammatory cytokines.