中药止痛巴布贴对骨癌痛大鼠痛行为及脊髓背角c-fos表达的影响

目的:观察中药止痛巴布贴对骨癌痛大鼠痛行为及脊髓背角神经节c-fos表达的影响。方法:108只180～220g雌性Wistar大鼠随机分为3组,分别为空白对照组(Con组,n=24)、假手术组(Sham组,n=24)、骨癌痛模型组(Ca组,n=60),3组分别于手术前2天、术后7、14、21日测定机械痛阈(MWT)和热痛阈(TWL)。于术后7天确认造模成功后,将成功的模型鼠随机分为两组,即模型组(Ca组,n=25)和中药止痛巴布贴组(CM组,n=18)。术后7、14和21天各组处死大鼠(n≥8),取大鼠脊髓腰椎L4-6膨大处,用免疫组化方法测定脊髓背角c-fos的变化。结果:MWT、TWL:术后Sham组、Ca组、CM组均低于基础值;术后7天,Sham组与基础值、Con组差异无统计学意义(P0.05),Ca组、CM组则低于基础值、Con组和Sham组(P0.05);术后14、21天Ca组与基础值、Con组和Sham组统计学差异逐渐增大(P0.01),而CM组则差异逐渐缩小(P0.05)。脊髓背角神经Fos表达:Ca组阳性神经元数目增加,术后7、14、21天与Con组和Sham组比较有统计学差异(P0.05);CM组于术后14、21天与Con组和Sham组比较统计学差异逐渐缩小(P0.05)。结论:中药止痛巴布贴对骨癌痛有比较明显的镇痛作用,其作用是通过影响脊髓背角神经细胞c-fos基因的表达而产生的。

Effect of Pain-Relieving Plaster on Pain Behaviors and c-Fos Expression in Spinal Dorsal Horn in Rats with Bone Cancer

Objective:The impacts of Chinese Traditional Medicine Pain-Relieving Plaster on the pain behaviors and the influence of the expression of c-fos in the spinal cord dorsal root ganglion of rats with bone cancer were observed.Method:108 female Wistar rats(180~220g)were randomly divided into 3 groups,blank control group(group Con,n=24),sham operation group(group Sham,n=24),bone cancer pain model group(group Ca,n=60).2 days before and 7,14,21 days after operation,mechanical pain threshold(MWT)and thermal pain threshold(TWL)in the 3 groups were tested.On the 7th day postoperatively confirmed after injecting,the success of the model rats were randomly divided into two groups,namely the model group(group Ca,n=28)and Chinese Traditional Medicine Pain-Relieving Plaster group(group CM,n=25).After 7 days,14 days and 21 days rats in each group were sacrificed(n≥8),rat spinal cord lumbar L4-6 was modified by immunohistochemical method,the changes of Fos in spinal dorsal horn were observed.Result:(1)MWT、TWL:the levels in Postoperative Sham group,Ca group,CM group were lower than the base value;7 days after operation,the levels in Sham group and Con group,combined with base value,the difference was not statistically significant(P>0.05);the levels in Ca group,CM group were lower than the base value and that in Con group and Sham group(P<0.05).But levels in group CM after 14,21 days,combined with the base and that in Con group and Sham group,the difference was increased(P<0.05);after 14,21 days,the difference between group Ca and basic value,Con group,Sham group were statistically significant increased gradually(P<0.01),while in CM group the differences become smaller(p>0.05).(2)Spinal cord dorsal horn neuronal Fos expression:positive neurons in group Ca increased,after 7,14,21 days,compared with in group Sham there was statistical difference(P<0.05);after 14,21 days,comparison between group CM group and Con and group Sham were statistically different gradually(P>0.05).Conclusion:Chinese Traditional Medicine Pain-Relieving Plaster has obvious analgesic effect,its role is through the effects on spinal dorsal root ganglion of the expression of Fos protein.