多发性骨髓瘤中p15基因高甲基化的研究

目的：探索p15（MST INK4b）基因在多发性骨髓瘤中的作用特点和甲基化发生的规律。方法：用甲基化特异的PCR的方法-MSP（methylation-specific PCR），检测23例多发性骨髓瘤（multiple myeloma, MM）p15基因甲基化的发生率。结果：MM中甲基化的发生率为73.5%(17/23)，扩增产物为148 bp片段。其中4例Ⅰ期，骨髓象为浆细胞型的MM p15基因未发生甲基化；1例Ⅱ期和1例Ⅲ期，骨髓象为分化成熟的浆细胞的MM中p15基因未发生甲基化；在Ⅰ、Ⅱ和Ⅲ期中多数为浆细胞型或混合型中的幼稚浆细胞的病例易发生甲基化。Ⅰ、Ⅱ期和Ⅲ期中甲基化的发生率分别为55%(5/9)、100%(7/7)和71.4%(5/7)。结论：p15基因甲基化可能是MM发病原因之一，与病程的进展和预后有一定的关系。

Study on hypermethylation of p15INK4B gene in multiple myeloma

AIM: To study the characteristics and regulations of p15 (MST INK4B) methylation in multiple myeloma (MM). METHODS: Method of methylation-specific PCR was applied in 23 cases of MM about the methylation rate of p15 gene. RESULTS: Methyaltion rate in MM was 73.5%(17/23). The PCR product was a fragment of 148 bp. In four stageⅠcases of MM with plasma cell-typed bone marrow profile, p15^INK4B gene was non-methylated; In one case of stag ⅡMM and one case of stage Ⅲ MM with mature plasma typed bone marrow profile, p15^INK4B gene was no-methylated, too, while in many cases of stageⅠ、stage Ⅱand stage Ⅲ with naive plasma cell in bone marrow profile which were plasma cell-typed or mixed typed, p15^INK4B gene methylation was frequently detected. The methylation rates for stageⅠ、stage Ⅱand stage Ⅲ MM were respectively 55%(5/9),100%(7/7) and 71.4%(5/7). CONCLUSION: p15 gene methylation was a possible pathogenic factor,and might be related to the progression and prognosis of the disease.