| 大肠埃希菌药敏分析及qnr基因分布情况研究 |
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| 引用本文： | 胡景玉,杜红丽,乔艳梅,郭连峰,杨世金,金玉怀.大肠埃希菌药敏分析及qnr基因分布情况研究[J].临床肺科杂志,2013,18(2):290-292. |
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| 作者姓名： | 胡景玉 杜红丽 乔艳梅 郭连峰 杨世金 金玉怀 |
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| 作者单位： | 河北医科大学附属哈励逊国际和平医院;河北医科大学病原生物学教研室 |
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| 摘 要： | 目的了解衡水地区大肠埃希菌的药敏情况以及qnr基因的存在情况。方法采用肉汤稀释法测15种抗菌药物最低抑菌浓度（MIC）并计算其MIC50和MIC90；采用聚合酶链反应（PCR）检测qnr基因。结果15种抗菌药物中仅亚胺培南、哌拉西林／他唑巴坦和头孢哌酮／舒巴坦对大肠埃希菌敏感，245例大肠埃希菌中检出5株存在qnrB基因，占2．O％，1株存在qnrS基因，占0．4％。结论我院检出的大肠埃希菌存在多重耐药。少数菌株中，存在qnrB和qnrS基因，临床应加强监测。
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| 关 键 词： | 衡水地区 MIC50 M1C90 qnr 菌落PCR |
Analysis of the antibiotics susceptibility and qnr gene distrabution on Escherichia coli isolated from clinical |
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| HU Jing-yu,DU Hong-li,QIAO Yan-mei,GUO Lian-feng,YANG Shi-jin,JIN Yu-huai.Analysis of the antibiotics susceptibility and qnr gene distrabution on Escherichia coli isolated from clinical[J].Journal of Clinical Pulmonary Medicine,2013,18(2):290-292. |
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| Authors: | HU Jing-yu DU Hong-li QIAO Yan-mei GUO Lian-feng YANG Shi-jin JIN Yu-huai |
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| Institution: | 1 Harrison In ternational Peace Hospital Aff iliated to HebeiM edical Un iversity,Hengshui 053000,P.R.China,2 Department of Pathogen Biology,Hebei Medical University |
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| Abstract: | Objective to understand the resistance characteristics as well as the distribution of qnr of E. eoli in Hengshui area. Methods Minimum inhibitory concentrations （MICs） to 15 antibiotics of the isolates were determined by broth dillution method, and the value of MICs0 and M｜C90 were calculated. Qnr genes were amplified by polymerase chain reaction （PCR）. Results imipenem, pip- eracillin/tazobaetam and eefoperazone/sulbaetam is highly sensitive to E. eoli, 5 isolates were found carrying qnrB gene among the 245 strains of isolates （2.0%） and 1 strain was qnrS positive （0.4%）. Conclusions Hospital detection of E. eoli multi-drug resistant. A snmll number of strains, there qnrB and qnrS genes, clinical, should be strengthened to monitor. |
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| Keywords: | Hengshui area MIC50 MIC90 qnr Colony PCR |
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