用异丙叉麦芽庚糖呋喃果糖苷(IPG7F)作为双封闭底物测定内切淀粉酶活力

作者用自己合成的邻-(4,6-邻-异丙叉-2-D-吡喃葡萄糖)-(1→4)-(邻-2-D-吡喃葡萄糖-(1→4)]_5-邻-2-D·吡喃葡萄糖-(1→2)-2-D-呋喃果糖苷,O-(4,6-O-isopropylidcno-2-D-glucopy-ranosyl)-(1→4)-[0-2-D-glucopyranosyl-(1→4)]_5-0-2-D-glucopyranosyl-(1→2)-2-D-fruc-tofuranosidc(IPG7F)作为淀粉酶底物,用葡萄糖淀粉酶、α-葡萄糖苷酶和甘露醇脱氢酶作为偶联酶,动态法测定淀粉酶活力,取得了满意的效果。