Phenotypic determination of T-lymphocytes responding to chemotactic stimulation from fMLP,IL-8, human IL-10, and epidermal lymphocyte chemotactic factor |
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Authors: | C O C Zachariae T Jinquan V Nielsen K Kaltoft K Thestrup-Pedersenl |
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Institution: | (1) Department of Dermatology, Marselisborg Hospital, DK-8000 Aarhus C, Denmark;(2) Institute of Human Genetics, University of Aarhus, DK-8000 Aarhus C, Denmark |
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Abstract: | Summary Human T lymphocytes were collected after they had migrated towards N-formyl-methionyl-leukyl-phenylalanine (fMLP), rIL-8, human IL-10 (hIL-10), and epidermal lymphocyte chemotactic factor (ELCF). They were stained for determination of their phenotype by FACS analysis using anti-CD4, -CD8, -CD18, -CD45R0 and OPD4 antibodies. Human IL-10 increased the percentage of CD8+ T lymphocytes in the migrating cell population by 152% compared with cells migrating towards the medium and decreased the number of CD4+ T lymphocytes by 79%. ELCF increased the number of CD4+ T lymphocytes by 18%, and the number of CD45R0+ T lymphocytes by 52%, while the number of CD8+ T lymphocytes was decreased by 20%. rIL-8 increased the number of CD4+ T lymphocytes and decreased the CD8+ T lymphocytes. The distribution of the different subpopulations of T lymphocytes was not changed significantly by fMLP. The observed changes in the phenotypes did not occur when incubating T lymphocytes with the chemotaxins. Our observations demonstrate that individual chemotactic factors will attract specific subsets of T lymphocytes. They may help to explain the predominance of memory T lymphocytes (CD4R0+, CD4+) in allergic contact dermatitis and certain other skin diseases. They also confirm the results of a recent study, that showed hIL-10 to be selectively chemotactic for CD8+ T lymphocytes. |
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Keywords: | Phenotype T lymphocytes Chemotaxis IL-8 IL-10 ELCF fMLP |
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