Effect of p120 catenin silencing on biological behaviors of PANC-1 cells |
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Authors: | Zhangjun Cheng Volker Assfag Xin Shi Shibo Lin Jiangyan Xia Pinghua Yang Norbert Hüser Feng Shen |
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Institution: | 1Department of General Surgery,the Affiliated Zhongda Hospital,Southeast University,Nanjing 210009,China 2Eastern Hepatobiliary Surgical Hospital,Second Military Medical University,Shanghai 200438,China 3Department of Surgery,Klinikum rechts der Isar,Technische Universitt München,Munich D-81675,Germany |
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Abstract: | This study examined the possible role of p120ctn in the pathogenesis and development of pan-creatic cancer.PANC-1 cells,a kind of human pancreatic carcinoma cell line,were cultured in this study.p120ctn was immunocytochemically detected in PANC-1 cells.The recombinant lentivirus vector was constructed to knock down the p120ctn expression of PANC-1 cells.Real-time quantitative PCR (RQ-PCR) and Western blotting were used to determine the expression of p120ctn and E-cadherin in PANC-1 cells after p120ctn knockdown.The adhesion,invasion and migration capacity of PANC-1 cells after p120ctn knockdown was detected by cell adhesion,invasion and migration assays.Cell growth was measured by the MTT method.Cell cycle and apoptosis were analyzed by fluorescence-activated cell sorting.The results showed that p120ctn knockdown led to significantly down-regulated E-cadherin and a reduced cell-to-cell adhesion ability in PANC-1 cells.shRNA-mediated knockdown of p120ctn reduced invasion and migration capacity of PANC-1 cells,inhibited cell growth,caused a significant decrease in the percentage of cells in G1,an increase in S,and promoted apoptosis of PANC-1 cells.It was concluded that p120ctn plays a pivotal role in the proliferation and metastasis of pancreatic carcinoma,suggesting that p120ctn is a novel target for pancreatic carcinoma treatment. |
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Keywords: | p120ctn pancreatic carcinoma invasion migration small hairpin RNA interference |
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