免疫组织化学染色在妇科盆腹腔积液细胞学诊断中的应用

目的探讨免疲组化染色在妇科腹腔脱落细胞学诊断中的应用价值。方法选取45例盆腹腔积液及腹腔冲洗液中有异型细胞的女性病例，对液体离心沉淀后，沉淀物石蜡包埋，切片后进行CK7，CK20，Calretinin，WT1，p53，ER，PR，CK5／6的免疫组织化学染色检测。结果在含有肿瘤细胞的阳性病例组中，CK7，WT1和p53有较高的阳性表达率，阳性率分别为89.3％，62．9％和68．4％。而在未找到肿瘤细胞的阴性病例组中，CK7，Calretinin和CK5，6阳性表达率较高，分别为72．7％，71．4％和57．1％。结论盆腹腔积液的沉淀细胞团块经包埋切片后，可以用于免疫组织化学染色，对于盆腹腔渗出液的细胞学诊断具有帮助。

Application of immunohistochemistry for cytological diagnosis in the gynecological pelvic and peritoneal cavity fluids

Objective To assess the diagnostic value of immunohistochemistry for cytological examination in the gynecological pelvic and peritoneal cavity effusions. Methods 45 cases of fluids from pelvic and peritoneal effusions of females were selected, which were observed atypical cells in routine cytological evaluation. Cell clot blocks were prepared by centrifuge and deposition. Immunohistochemistry for CK7, CK20, Calretinin, WT1, p53, ER, PR, CK5/6 were performed on cell blocks sections. Results CK7, WT1 and p53 showed high proportion of positive expression in cases including atypical/malignant cells, which positive rate were 89.3%, 62.9% and 68.4% respectively. CK7, Calretinin and CK5/6 showed high proportion of positive expression in cases negative for malignancy, which positive rate were 72.7%,71.4% and 57.1% respectively. Conclusion Clot preparation from pelvic and peritoneal cavity fluids can be used immunohistochemistry by block and section. It is help to cytological diagnosis of pelvic and peritoneal effusions.