| Effects of Novel recombination human tumor necrosis factor on tumor cell growth and NF-KB activity |
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| 作者姓名： | 孟洁如 赵宁 颜真 李玉松 王文勇 常耀明 张英起 |
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| 作者单位： | Biotechnology Centre，Faculty of Preclinical Medicine，Fourth Military Medical University，Xi'an 710033，China，Biotechnology Centre，Faculty of Preclinical Medicine，Fourth Military Medical University，Xi'an 710033，China，Biotechnology Centre，Faculty of Preclinical Medicine，Fourth Military Medical University，Xi'an 710033，China，Department of Pathology，Faculty of Preclinical Medicine，Fourth Military Medical University，Xi'an 710033，China，Department of Pathology，Faculty of Preclinical Medicine，Fourth Military Medical University，Xi'an 710033，China，Faculty of Aviation Medicine，Faculty of Preclinical Medicine，Fourth Military Medical University，Xi'an 710033，China，Biotechnology Centre，Faculty of Preclinical Medicine，Fourth Military Medical University，Xi'an 710033，China |
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| 摘 要： | Objective: To investigate the relationship among the TNFRp55 and TNFRp75 expression in tumor cell lines in vitro, the suppression of nrhTNF on the tumor cell lines growth and the changing of NF-kB activity in the tumor cell lines. Methods:The expressions of TNFRs are studied by immunohistochemistry. The inhibition of nrhTNF to tumor cells was investigate by crystal violet assay. The changing of activity of NF-icB was evaluated by using immunohistochemistry and Western blot methods. Results: In tumor cell lines, positive rates of expression of TNFRp55 and TNFRp75 were 99% and 98. 8% respectively. There was no significant difference among various types of tumor cells (P>0. 05). nrhTNF could inhibit the growth of SW480, SGC7901, 8910, SMMC7721 and BEL-7402 cell lines in which NF-icB translocated into cytonuclei slightly. However, nrhTNF has no effects on A549, PLA801, Hela, Ecal09 and GRC-1 cell lines in which NF-kB translocated into cytonuclei obviously. Conclusion: The low degree of activating of NF-kB m
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| 关 键 词： | NF-κB |
Effects of Novel recombination human tumor necrosis factor on tumor cell growth and NF-kB activity |
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| MENG Jie-Ru,ZHAO Ning,YAN Zhen,LI Yu-song,WANG Wen-Yong,Chang Yao-ming,ZHANG Ying-Qi.Effects of Novel recombination human tumor necrosis factor on tumor cell growth and NF-KB activity[J].Journal of Medical Colleges of PLA(China),2002,17(3). |
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| Authors: | MENG Jie-Ru ZHAO Ning YAN Zhen LI Yu-song WANG Wen-Yong Chang Yao-ming ZHANG Ying-Qi |
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| Abstract: | Objective: To investigate the relationship among the TNFRp55 and TNFRp75 expression in tumor cell lines in vitro, the suppression of nrhTNF on the tumor cell lines growth and the changing of NF-kB activity in the tumor cell lines. Methods:The expressions of TNFRs are studied by immunohistochemistry. The inhibition of nrhTNF to tumor cells was investigate by crystal violet assay. The changing of activity of NF-icB was evaluated by using immunohistochemistry and Western blot methods. Results: In tumor cell lines, positive rates of expression of TNFRp55 and TNFRp75 were 99% and 98. 8% respectively. There was no significant difference among various types of tumor cells (P>0. 05). nrhTNF could inhibit the growth of SW480, SGC7901, 8910, SMMC7721 and BEL-7402 cell lines in which NF-icB translocated into cytonuclei slightly. However, nrhTNF has no effects on A549, PLA801, Hela, Ecal09 and GRC-1 cell lines in which NF-kB translocated into cytonuclei obviously. Conclusion: The low degree of activating of NF-kB makes tumor cells sensitive to nrhTNF, however, the high degree of activating of NF-kB makes tumor cells insensitive to nrhTNF. |
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| Keywords: | tumor cells TNFRp55 TNFRp75 nrhTNF NF-kB |
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