格林——巴利综合征患者的空肠弯曲菌抗体及意义

目的：用纯化的脂多糖抗原（CJ-LPS）和全菌抗原（CJ-T）检测格林-巴利综合征（GBS）患者血清的空肠弯曲菌（CJ）抗体,并用2种抗原检测到的抗体阳性率和阳性病例的符合率的差别来推测CJ的LPS是否为诱发CJ抗体的抗原表位。方法：取GBS患者分离的15株不同Penner血清型的CJ,用酚-水法提取的CJ-LPS抗原,以热稳定成分为CJ-T抗原,以ELISA间接法检测81例GBS患者、34例其他神经疾病对照和63例正常对照的血清CJ抗体。结果：CJ-LPS为抗原,CJ感染阳性率为51.9%,CJ-T检测的阳性率为56.8%,与其他神经疾病对照和正常对照的差异均有显著性。2种抗原检测得到的阳性率之间差异没有显著性,阳性病例的符合率之间差异没有显著性。结论：我国GBS患者的CJ近期感染阳性率较高,推测CJ上的LPS可能为诱发GBS的一类表位抗原。

Serum antibody to Campylobacter jejuni(CJ) in Guillain-Barre Syndrome(GBS) patients and its significance

Objective:To detect serum antibody to CJ in GBS patients with LPS purified from CJ strains (CJ LPS) and total CJ constituents (CJ T) as antigens and to deduce whether LPS is surely the epitope of CJ in the induction of CJ antibodies from the difference between the positive rates of both the two assays with CJ LPS and CJ T and from the concordance rates of the positive cases Methods:Sera from 81 GBS patients, 34 patients with other neurological diseases (OND) and 63 normal controls (NC) were tested with CJ LPS and CJ T by indirect ELISA method CJ LPS was purified by phenol water extraction method from 15 CJ strains of various Penner serotypes, including those isolated from GBS patients CJ T was made by collecting the heat stable constituents of CJ Results:The positively infective rate of GBS were 51 9% by CJ LPS and 56 8% by CJ T antigens There is significant difference between GBS and NC in both assays, as well as OND There is no difference between the positively infective rate of GBS patients detected with the two sets of antigens and between concordance rates of the positive cases Conclusion:High incidence of GBS following CJ infection was confirmed once more, and LPS is probably one of the epitopes of CJ in the induction of CJ antibodies