左旋肉碱对高糖条件下人腹膜间皮细胞增殖、凋亡及纤维连接蛋白分泌的影响

目的：探讨左旋肉碱对高糖条件下体外培养人腹膜间皮细胞增殖、凋亡及纤维连接蛋白（FN）分泌的影响。方法：以不同浓度左旋肉碱作用体外培养人腹膜间皮细胞，MTT法测定细胞增殖，Annexin V-FITC／PI双标记法检测细胞凋亡，ELISA法检测培养液中FN浓度。结果：不同浓度左旋肉碱作用24h后，0．1％左旋肉碱组细胞增殖活性显著升高（P〈0．05），作用72h后0．5％及1．0％左旋肉碱组细胞增殖活性明显降低（P〈0．05）。作用48及72h后0．05％及0．1％左旋肉碱组细胞凋亡率降低。作用24h后0．05％左旋肉碱组培养液中FN浓度升高（P〈0．05），作用72h后左旋肉碱组培养液中FN浓度均下降（P〈0．01）。结论：高糖条件下一定浓度的左旋肉碱可以促进人腹膜间皮细胞增殖、减少其凋亡。

Effects of L-carnitine on proliferation, apoptosis, and fibronectin secretion of human peritoneal mesothelial cells under high glucose condition

Objective: To investigate the effect of L-carnitine on the proliferation,apoptosis,and fibronectin secretion of human peritoneal mesothelial cells(HPMC) under high glucose condition.Methods: HPMC derived from human greater omentum were cultured in vitro,and different concentrations of L-carnitine were added in the experiment group.The proliferation,apoptosis,and fibronectin concentration in the culture medium were determined by MTT colorimetric assay,Annexin V-FITC/PI double labeled assay,and enzyme-linked immunosorbent assay(ELISA),respectively.Results: The proliferation of HPMC significantly increased in 0.1% L-carnitine group 24 hours after the administration of L-carnitine(P<0.05),and significantly decreased in 0.5% and 1.0% L-carnitine groups 72 hours after the administration(P<0.05).The apoptosis of HPMC in 0.05% and 0.1% L-carnitine groups significantly decreased 48 and 72 hours after the administration(P<(0.01)).The concentration of fibronectin in 0.05% L-carnitine group significantly increased 24 hours after the administration(P<0.05),and decreased in each group 72 hours after the administration(P<0.01).Conclusion: L-carnitine of certain concentration can promote the proliferation and inhibit the apoptosis of HPMC under high glucose condition.