前脂肪细胞在小肠黏膜下层的生长与增殖

背景：研究表明前脂肪细胞能定向分化为脂肪细胞，具有很强的增殖能力。小肠黏膜下层具有良好的生物相容性，是理想的组织工程支架材料。 目的：将体外培养的前脂肪细胞与小肠黏膜下层复合，观察前脂肪细胞在小肠黏膜下层上的生长和增殖情况。 方法：将体外培养的前脂肪细胞接种在小肠黏膜下层上作为材料组，设置在孔板内培养的细胞作为对照组，在不同时间点用MTT法检测前脂肪细胞的相对数量并绘制生长曲线，观察前脂肪细胞在不同培养条件下的增殖能力。流式细胞仪检查两组前脂肪细胞的增殖和凋亡情况，并观察不同时间点前脂肪细胞生长和黏附情况。 结果与结论：从生长曲线可以看出，接种13 d后小肠黏膜下层上的前脂肪细胞数高于孔板内培养的前脂肪细胞数(P < 0.05)，具有良好的增殖能力。小肠黏膜下层上的细胞比孔板培养的细胞有更强的增殖能力(P < 0.05)，两组前脂肪细胞均无明显凋亡。由此可知，前脂肪细胞在小肠黏膜下层具有良好黏附和生长的能力。小肠黏膜下层与前脂肪细胞有良好的生物相容性。 关键词：前脂肪细胞；小肠黏膜下层；细胞增殖；生长；黏附；组织工程；生物相容性 doi:10.3969/j.issn.1673-8225.2010.25.010

Growth and proliferation of preadipocyte seeded on small intestinal submucosa

BACKGROUND: The preadipocytes could differentiate into adipocyes with strong proliferation capacity. Small intestinal submucosa (SIS) has good biocompatibility, could be suitable scaffold for tissue engineering. OBJECTIVE: To culture preadipocytes in vitro on SIS, and to study the growth and proliferation of preadipocytes on SIS. METHODS: The preadipocytes cultured were respectively seeded on SIS and pore plates, serving as material group and control group. Cell number in different periods was determinded by MTT, and the growth curve was rendered. The proliferation capacity of the preadipocytes was observed under different culture conditions. Flow cytometry was used to detect the proliferation and apoptosis of preadipocytes seeded on SIS and pore plates. Preadipocytes on SIS were also observed for the growth and proliferation at different time points. RESULTS AND CONCLUSION: The growth curve showed that, the preadipocytes on SIS grew rapidly and the cell number was higher than that in pore plates on day 13 (P < 0.05), suggesting a strong proliferation capacity. Cells on SIS exhibited stronger proliferative capacity than those in pore plate (P < 0.05). Apoptotic rates were low in two groups without statistical difference. The preadipocytes are shown to well adhere and grow on SIS. SIS is very biocompatible with preadipocytes.