糖尿病患者庚型肝炎病毒感染及其临床意义

目的　了解糖尿病患者 (I型和II型 )庚型肝炎病毒 (HGV)感染情况及临床意义。方法　应用逆转录套式聚合酶链反应 (RT -NestedPCR)检测 183例糖尿病患者血清标本中的HGVRNA ,部分扩增产物进行核苷酸序列分析 ,所有患者均进行ALT检查。结果　I型和II型糖尿病患者血清标本中HGV检出阳性率分别为 4 6 7% (7/ 15 )和 2 0 2 % (34/ 16 8) ,总阳性率为 2 2 4 % (41/ 183) ;5例HGV 5’ -NCR和NS3区扩增产物与文献报道的核苷酸序列同源性分别为 87 2 %～ 89 4 %和 79 8%～ 83 5 % ;HGV感染的糖尿病患者ALT异常 (5 2～ 88U)的比例 (7 1% ,13/ 183)明显高于未感染HGV的糖尿病患者 (1 1% ,2 / 183)。结论　糖尿病患者 ,尤其是I型糖尿病患者的HGV感染率较高 ;不同地区检出的HGV基因组RNA片段的核苷酸序列存在一定的差异 ;HGV感染可导致部分患者轻微的肝脏损害。

Hepatitis G virus infection in diabetes patients and its clinical signifincance

Aim To understand the frequency of Hepatitis G virus (HGV)infection in diabetes patients (type I and type II)and clinical significance of the infection Methods HGV RNA in the serum samples from 183 diabetes patients was detected by RT nested PCR and amplification products from part of the serum samples with HGV RNA were sequenced ALT examination in the 183 serum samples was also performed Results 46 7%(7/15)of the serum samples from the patients with diabetes type I and 20 2%(34/168)of the serum samples from the patients with diabetes type II were positive for HGV RNA at 5' NCR and NS3 regions respectively The total positive rate of HGV RNA in these patients was 22 4% (41/183) In comparison with the reported HGV genome sequence,the homologies of the nucleotide sequences of RT nested PCR products from HGV 5' NCR and NS3 region from 5 serum samples were 87 2%～89 4% and 79 8%～83 5%,respectively The proportion of ALT elevation (52～88U)in the serum samples with HGV RNA positive (31 7%,13/41)was statistically higher than those with HGV RNA undetectable (1 4%,2/142)(P<0 01) Conclusion The infection rates of HGV in diabetes patients,especially in diabetes type I patients,are relatively high The genomic heterogeneity of HGV genome is present in isolates from different areas of the world HGV infection in the part of diabetes patients is able to result in mild liver injury