| 猫爪草粗多糖提取工艺研究 |
| |
| 引用本文： | 刘菊,王君明,纪彬,张红伟,赵群涛. 猫爪草粗多糖提取工艺研究[J]. 河南中医学院学报, 2012, 27(12): 1622-1623 |
| |
| 作者姓名： | 刘菊 王君明 纪彬 张红伟 赵群涛 |
| |
| 作者单位： | 1. 信阳市食品药品检验所,河南信阳,464000
2. 河南中医学院,河南郑州,450046 |
| |
| 基金项目： | 基金项目：河南中医学院博士科研基金（编号：BSJJ2010-22） |
| |
| 摘 要： | 目的:优选猫爪草粗多糖提取工艺.方法:采用苯酚-硫酸法测定粗多糖含量(以葡萄糖计),通过正交设计L9(34)优化水提和醇沉工艺.结果:猫爪草粗多糖最佳水提工艺为A3B2C3,即加12倍量水,提取2次,每次3h;最佳醇沉工艺为A1B1C3,即提取液浓缩至800g·L-1,加乙醇使含醇量达50％,静置6h.结论:该方法制备猫爪草粗多糖操作简便、经济、无毒,适用于工业化生产.
|
| 关 键 词： | 猫爪草粗多糖 中药成分提取 正交设计 工艺优化 |
Research on Extraction Process of Crude Polysaccharide from Ranunculus Ternatus Thunb |
| |
| Liu Ju,Wang Junming Ji Bin,Zhang Hongwei,Zhao Quntao. Research on Extraction Process of Crude Polysaccharide from Ranunculus Ternatus Thunb[J]. Journal of Henan University of Chinese Medicine, 2012, 27(12): 1622-1623 |
| |
| Authors: | Liu Ju Wang Junming Ji Bin Zhang Hongwei Zhao Quntao |
| |
| Affiliation: | (Food and Drug Control Institute of Xinyang, Xinyang, Henan, China 464000;Henan University of Traditional Chinese Medicine, Zhengzhou, Henan, China 450046) |
| |
| Abstract: | Objective：To optimize the best extraction process of crude polysaccharide from Ranunculus ternatus thunb. Methods：Phenol- sulfuric acid method was used to determine the content of crude polysaccharide （based on the content of glucose）, orthogonal design L9 （34 ） was used to optimize the best extraction and alcohol precipitation process of polysaccharide. Results ： The best extraction process of crude polysaccharide from Ranunculus ternatus thunb was A3B2C3 ,namely extracting twice with adding 12 times water,3 hours every time;the best alcohol precipitation Process was A1B1C3, namely concentrating extraction solution to 0.8 g · mL^-1 , adding ethanol to make the content of ethanol be 50% with standing 6 hours. Conclusion ：This method of preparing crude polysaccharide from ranunculus ternatus thunb is simple, economic, non-toxic, and is suitable for industrial mass production. |
| |
| Keywords: | crude polysaccharide from Ranunculus tematus thunb Chinese medicinal ingredients extraction orthogonal design processoptimization |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
