川芎挥发油的GC-MS指纹图谱研究

目的:建立川芎挥发油的气相色谱-质谱(GC-MS)指纹图谱。方法:采用超临界CO2流体萃取法提取川芎挥发油,用GC-MS联用技术对其进行指纹图谱研究,色谱柱为SGE-30QC3/AC225弹性石英毛细管柱(30m×0.32mm×0.25μm),进样口温度为280℃,程序升温,载气为氦气,进样量为1μL,分流比为10:1;采用电子轰击离子源(EI),离子源温度为200℃,接口温度为250℃,检测电压为0.8kV,质量扫描范围为30～400amu;并采用"中药指纹图谱计算机辅助相似度评价软件"计算相似度。结果:本方法有较好的重复性、精密度和稳定性(RSD均<3%);川芎挥发油主要含有α-蒎烯、胡萝卜烯醇、川芎内酯、藁本内酯、棕榈酸,这5种成分构成了川芎挥发油GC-MS特征指纹图谱;10批川芎挥发油的相似度均>0.92。结论:本研究可为川芎的质量控制提供依据。

Study on the Fingerprints of Volatile Oil from Ligusticum chuanxiong by GC-MS

OBJECTIVE：To establish GC-MS fingerprints of volatile oil from Ligusticum chuanxiong.METHODS：The volatile oil was extracted from L.chuanxiong by supercritical CO2 fluid extraction.The chromatographic fingerprints was established by GC-MS,SGE-30QC 3/AC 225 fused-silica capillary（30 m×0.32 mm×0.25 μm） column,injector temperature of 280 ℃,temperature programming,carrier gas of helium,injection volume of 1 μL,split ratio of 10：1,ion source of EI,ion source temperature of 200 ℃,interface temperature of 250 ℃,detection voltage of 0.8 kV,scanning range of ion mass from 30 amu to 400 amu.The similarity was calculated with ＂TCM fingerprint computer aided similarity evaluation sofetware＂.RESULTS：The reproducibility,accuracy and stability of the method were very good in the tests（all RSD3%）;the volatile oil from L.chuanxiong mainly included α-pinene,carotol,Chuanxiong lactone,ligustilide and palmitic acid.They made up of GC-MS fingerprints of volatile oil from L.chuanxiong;the similarity of volatile oil from 10 batches of L.chuanxiong was more than 0.92.CONCLUSION：The study can provide reference for quality control of L.chuanxiong.