大鼠灌胃给予维血宁颗粒后虎杖苷血药浓度测定及药动学研究

目的:建立大鼠血浆中虎杖苷浓度的反相高效液相色谱(RP-HPLC)测定方法。方法:色谱柱为Diamonsil C18(200mm×4.6mm,5μm),流动相为乙腈-水(20∶80,V/V),流速为1.0mL·min-1,柱温为35℃,检测波长为303nm。大鼠ig维血宁颗粒后,测定不同时间血药浓度。采用DAS2.0药动学软件对血药浓度-时间数据进行拟合,计算相应药动学参数。结果:大鼠灌胃给予维血宁颗粒后,虎杖苷在大鼠体内药动学主要参数为:tmax=(0.403±0.063)h,Cmax=(1.715±0.097)mg·L-1,Ka=(6.894±3.275)h-1,t1/2α=(0.202±0.142)h,t1/2β=(2.484±1.624)h,CL/F=(32.229±22.027)L·h-1·kg-1,V1/F=(14.447±12.013)L·kg-1,AUC(0～t)=(1.511±0.550)mg·h·L-1,AUC(0～∞)=(1.955±0.765)mg·h·L-1。结论:虎杖苷在大鼠体内的药动学过程符合二室模型,进入体内分布迅速,代谢消除速度也较快。

Determination of Polydatin in Rat Plasma and The Pharmacokinetics Study After Oral Administration of Weixuening Granule

OBJECTIVE：To establish an RP-HPLC method for the determination of polydatin in rat plasma and its pharmacokinetic study in vivo after ig.METHODS：The analysis was performed on a Diamonsil C18（200 mm×4.6 mm,5 μm） column,with the acetonitrile-water（20：80,V/V）as the mobile phase.The flow rate was 1 mL·min-1,and the detective wave length was set at 303 nm.The Weixuening granule was administrated by i.g,and blood samples were collected a different time and detected.The data were calculated by DAS2.0 software.RESULTS：The primary pharmacokinetic parameters of polydatin were as follows：tmax=（0.403±0.063） h,Cmax=（1.715±0.097） mg·L-1,Ka=（6.894±3.275） h-1,t1/2α=（0.202±0.142） h-1,t1/2β=（2.484±1.624） h-1,CL/F=（32.229±22.027） L·h-1·kg-1,V1/F=（14.447±12.013） L·kg-1,AUC（0～t）=（1.511±0.550） mg·h·L-1 and AUC（0～∞）=（1.955±0.765） mg·h·L-1.CONCLUSION：The pharmacokinetic behavior of polydatin in rats was fitted to two-compartment open model,and it is distributed and eliminated quickly in vivo.