| RP-HPLC法测定三皮止癣酊中蛇床子素的含量 |
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| 引用本文： | 邹渭洪,付成效,龙宇,刘琪.RP-HPLC法测定三皮止癣酊中蛇床子素的含量[J].中国药房,2012(47):4484-4485. |
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| 作者姓名： | 邹渭洪 付成效 龙宇 刘琪 |
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| 作者单位： | [1]南华大学附属第一医院药剂科,湖南衡阳421001 [2]南华大学附属第二医院药剂科,湖南衡阳421001 [3]衡阳市中医院药剂科,湖南衡阳421001 |
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| 摘 要： | 目的:建立测定三皮止癣酊中蛇床子素含量的方法。方法:采用反相高效液相色谱法。色谱柱为Ultimate XB-C1(8250mm×4.6mm,5μm),流动相为甲醇-水(76∶24,V/V),流速为1.0mL·min-1,检测波长为322nm,柱温为25℃。结果:蛇床子素进样量在0.0384～0.7680μg范围内与峰面积积分值呈良好线性关系(r=0.9996);平均加样回收率为100.45%,RSD=1.88%(n=6)。结论:本方法操作简便、快速、准确,适用于三皮止癣酊中蛇床子素的含量测定。
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| 关 键 词： | 三皮止癣酊 反相高效液相色谱法 蛇床子素 含量测定 |
Content Determination of Osthole in Sanpi Zhixuan Tincture by RP-HPLC |
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| ZOU Wei-hong,FU Cheng-xiao Dept. of Pharmacy,Hengyang,China The First Affiliated Hospital of Nanhua University,Hunan LONG YuDept. of Pharmacy,The Second Affiliated Hospital of Nanhua University,Hunan Hengyang,China LIU Qi.Content Determination of Osthole in Sanpi Zhixuan Tincture by RP-HPLC[J].China Pharmacy,2012(47):4484-4485. |
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| Authors: | ZOU Wei-hong FU Cheng-xiao Dept of Pharmacy Hengyang China The First Affiliated Hospital of Nanhua University Hunan LONG YuDept of Pharmacy The Second Affiliated Hospital of Nanhua University Hunan Hengyang China LIU Qi |
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| Institution: | ZOU Wei-hong, FU Cheng-xiao (Dept. of Pharmacy, Hengyang 421001, China) The First Affiliated Hospital of Nanhua University, Hunan LONG Yu(Dept. of Pharmacy, The Second Affiliated Hospital of Nanhua University, Hunan Hengyang 421001, China) LIU Qi(Dept. of Pharmacy,Hengyang Hospital of Traditional Chinese Medicine,Hunan Hengyang 421001 ,China) |
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| Abstract: | OBJECTIVE： To establish a method for the content determination of osthole in Sanpi zhixuan tincture. METHODS： RP-HPLC method was adopted. The determination was performed on Ultimate XB-C18（250 minx4.6 mm, 5 μm） column with mobile phase consisted of methanol-water（76 ： 24, V/V） at the flow rate of 1.0 mL.min^-1. The detection wavelength was set at 322 nm and column temperature was 25 ℃. RESULTS： The linear range of 0sthole was 0.038 4-0.768 0 μg（r=0.999 6）with an average recovery of 100.45 % （RSD=1.88 %, n = 6）. CONCLUSION： The method is simple, rapid, accurate and suitable for the content determination of osthole in Sanpi zhixuan tincture. |
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| Keywords: | Sanpi zhixuan tincture RP-HPLC Osthole Content determination |
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