| 广州胃病患者幽门螺杆菌多株感染的研究 |
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| 引用本文： | 曾志荣,陈湖,胡品津,崔毅,陈为,彭晓忠.广州胃病患者幽门螺杆菌多株感染的研究[J].世界华人消化杂志,1999,0(2):R322. |
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| 作者姓名： | 曾志荣 陈湖 胡品津 崔毅 陈为 彭晓忠 |
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| 作者单位： | 中山医科大学附属一院消化内科,广东省,广州市,510080 |
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| 基金项目： | 高等学校博士点基金,广东省科委自然科学基金 |
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| 摘 要： | 目的了解我国Ｈｐ感染人群中的多株感染状况．方法取因上腹不适行内镜检查的２０例Ｈｐ阳性患者（胃溃疡６例，十二指肠溃疡８例，慢性胃炎６例）的胃窦和胃体粘膜组织，进行Ｈｐ培养．初代培养后，分别取胃窦和胃体各１０个菌落进行传代，抽提各菌落ＤＮＡ，应用聚合酶链反应随机引物扩增的ＤＮＡ多态指模技术［ＲＡＰＤＰＣＲ］进行菌株鉴定．结果ＰＣＲ扩增产物电泳分析显示有１８例患者胃窦和胃体的ＨｐＤＮＡ指模一致，提示这１８例患者均是单株感染；另有２例胃体菌株表现为两种指模形态，提示为２株Ｈｐ感染．结论广州地区存在Ｈｐ多株感染，但不普遍．
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| 关 键 词： | 螺杆菌 幽门 胃溃疡 十二指肠溃疡 胃炎 胃镜检查 |
| 修稿时间： | 1998-06-06 |
Multiple strains of Helicobacter pylori infection in patients with gastropathy in Guangzhou |
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| ZENG Zhi-Rong,CHEN Min-Hu,HU Pin-Jin,CUI Yi,CHEN Wei,PENG Xiao-Zhong.Multiple strains of Helicobacter pylori infection in patients with gastropathy in Guangzhou[J].World Chinese Journal of Digestology,1999,0(2):R322. |
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| Authors: | ZENG Zhi-Rong CHEN Min-Hu HU Pin-Jin CUI Yi CHEN Wei PENG Xiao-Zhong |
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| Abstract: | AIM To
study multiple strains of Helicobacter pylori infection in Chinese subjects. METHODS H.
pylori isolated from 20 patients with gastropathy (gastric ulcer, 6 patients; duodenal ulcer, 8;
and chronic gastritis, 6) were analyzed by PCR based RAPD fingerprinting. Two biopsy
samples taken from the gastric antrum and body were cultured, and generations of 20 colonies
of H. pylori per subject (antrum 10 colonies, body 10 colonies) were analyzed. RESULTS In
18 patients, DNA fingerprinting patterns of all colonies were identical, but 2 patients had two
patterns in gastric body. CONCLUSION Most H. pylori infection is of single strain, and
multiple strain infection is not popular in Guangzhou area. |
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| Keywords: | Helicobacter pylori stomach ulcer duodenal ulcer gastritis gastroscopy |
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