| 高效液相色谱内标法测定无极膏中丙酸倍氯米松的含量 |
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| 引用本文： | 堵伟锋,李磊,马洪怡. 高效液相色谱内标法测定无极膏中丙酸倍氯米松的含量[J]. 安徽医药, 2009, 13(2): 162-163 |
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| 作者姓名： | 堵伟锋 李磊 马洪怡 |
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| 作者单位： | 安徽省食品药品检验所,安徽,合肥,230051;蚌埠市食品药品检验所,安徽,蚌埠,233000 |
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| 摘 要： | 目的建立测定无极膏中丙酸倍氯米松含量的方法。方法采用C18柱（4．6mm×250mm，5um），以甲醇·水（75：25）为流动相，流速为1．0ml·min^-1，检测波长240nm，用内标法测定，内标物为醋酸氯地孕酮。结果丙酸倍氯米松在2．09—41．76mg·L^-1范内呈良好线性关系（r=1），平均回收率为100．96％，RSD为1．6％。结论该方法准确，可靠，可用于无极膏的质量控制。
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| 关 键 词： | 高效液相色谱法 无极膏 丙酸倍氯米松 含量测定 |
Determination of beclomethasone dipropionate in cremor mentholi compositus by HPLC with internal standard |
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| DU Wei-feng,LI Lei,MA Hong-yi. Determination of beclomethasone dipropionate in cremor mentholi compositus by HPLC with internal standard[J]. Anhui Medical and Pharmaceutical Journal, 2009, 13(2): 162-163 |
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| Authors: | DU Wei-feng LI Lei MA Hong-yi |
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| Affiliation: | DU Wei-feng1,LI Lei1,MA Hong-yi2(1.Anhui Institute for Food , Drug Control,Hefei 230051,2.Bengbu Institute for Food , Drug Control,Bengbu 233000) |
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| Abstract: | Aim To establish a method for determination of beclomethasone dipropionate in cremor mentholi compositus. Methods C18 column（4.6 mm × 250 mm,5 um）was used, with methanol-water（75：25 ） as mobile phase. The flow rate was 1.0 ml · min^-1, and the wavelength of detector was 240 nm. The internal standard was chlormadinone acetate. Results The linear range of beclomethasone dipro- pionate was from 2.09 to 41.76 mg · L^-1 （ r = 1 ）. The average recovery was 100.96% （ RSD = 1.6% ）. Conclusion This method was proved to be simple and reliable, and can be used for the quality control of cremor mentholi compositus. |
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| Keywords: | HPLC cremor mentholi compositus beclomethasone dipropionate determination |
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