| 生殖细胞的细胞膜渗透特性研究进展 |
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| 作者姓名： | Zhou Z Chen G Zhang S |
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| 作者单位： | 浙江大学制冷与低温研究所 |
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| 基金项目： | 浙江省自然科学基金资助项目(Y1090409) |
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| 摘 要： | 生殖细胞的低温保存在多个领域具有重要应用价值。为提高保存后细胞的存活率与活性,研究细胞膜对水和低温保护剂的渗透特性十分必要。本文综述了近年来动物生殖细胞的细胞膜渗透特性研究概况,列出了典型的精子细胞和卵母细胞的细胞膜对水和常见低温保护剂的渗透系数,分析了这些细胞的渗透特性对制定其低温保存方案的影响。论文还介绍了细胞膜渗透特性的最新测量方法。
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| 关 键 词： | 生殖细胞 低温保存 细胞膜 渗透特性 测量方法 |
Progress with research on the permeability characteristics of reproductive cell membranes |
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| Zhou Z,Chen G,Zhang S.Progress with research on the permeability characteristics of reproductive cell membranes[J].Journal of Biomedical Engineering,2012,29(2):383-386. |
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| Authors: | Zhou Zheng Chen Guangming Zhang Shaozhi |
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| Institution: | Institute of Refrigeration and Cryogenics, Zhejiang University, Hangzhou 310027, China. |
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| Abstract: | The successful cryopreservation of reproductive cells has important practical significance in many fields. In order to improve the recovery rate and viability of cryopreserved cells, it is necessary to study the permeability characteristics of cell membrane to both water and cryoprotectant. In this paper we review the studies on membrane permeability of animal reproductive cell for the recent years. We firstly list the typical permeability data of spermatozoa and oocyte membrane for water and cryoprotectant. We then analyze the effects of these characteristics on the design of cryopreservation protocol. We also introduce the latest experimental methods to measure the cell membrane permeability. |
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| Keywords: | Reproductive cell Cryopreservation Cell membrane Membrane permeability Measure method |
| 本文献已被 CNKI PubMed 等数据库收录！ |
