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金雀异黄酮对体外培养成骨细胞抗缺氧的机制研究
引用本文:Han GQ,Ge BF,Chen KM,Ma HP. 金雀异黄酮对体外培养成骨细胞抗缺氧的机制研究[J]. 中药材, 2012, 35(1): 87-94
作者姓名:Han GQ  Ge BF  Chen KM  Ma HP
作者单位:兰州军区兰州总医院骨科研究所;兰州军区兰州总医院药材科
基金项目:甘肃省科技重大专项计划资助项目(092nkDA025)
摘    要:目的:观察金雀异黄酮对缺氧成骨细胞的抗缺氧作用。方法:以大鼠成骨细胞为材料,用三气培养箱建立缺氧模型。设常氧对照组、缺氧对照组和含不同浓度金雀异黄酮的缺氧加药组。比较各组缺氧36 h后细胞活力、ROS含量、细胞凋亡、细胞周期、PCNA表达i、NOS活性和钙化结节面积等,荧光定量PCR检测HIF-1α、BCL-2及Caspase-3 mRNA的表达。结果:金雀异黄酮显著提高缺氧成骨细胞存活率、G1期细胞百分比、钙化结节面积、HIF-1α和BCL-2 mRNA的表达水平,降低凋亡、ROS含量、PCNA表达量i、NOS酶活性和Caspase-3 mRNA表达。结论:金雀异黄酮能保护缺氧成骨细胞并促进其分化。

关 键 词:缺氧诱导因子-1α  金雀异黄酮  成骨细胞  细胞周期  凋亡  增殖细胞核抗原  活性氧簇

Study on mechanism of the anti hypoxia effect of genistein on osteoblasts in vitro
Han Gui-Qiu,Ge Bao-Feng,Chen Ke-Ming,Ma Hui-Ping. Study on mechanism of the anti hypoxia effect of genistein on osteoblasts in vitro[J]. Journal of Chinese medicinal materials, 2012, 35(1): 87-94
Authors:Han Gui-Qiu  Ge Bao-Feng  Chen Ke-Ming  Ma Hui-Ping
Affiliation:Institute of Orthopedics, Lanzhou General Hospital, Lanzhou Command, PLA. Lanzhou 730050, China. jnhanqiu@163.com
Abstract:Objective:To study the protective effect of genistein on osteoblasts treated with hypoxia.Methods:Rat osteoblasts were isolated from calvarias of newborn Sprague-Dawly rat by enzyme digestion and hypoxic environment was made by triple-gases incubator.Rat osteoblasts treated with hypoxia for 36 haurs.After 36 hours,cell viability,content of reactive oxygen species(ROS),analysis of cellular cycle and apoptosis,expression of proliferating cell nuclear antigen(PCNA),activity of iNOS and area of calcified nodules were detected.Total RNA was isolated and the gene expression of hypoxia inducible factor-1α(HIF-1α),BCL-2 and Caspase-3 was investigated by Real Time RT-PCR.Results:Genistein could significantly improve cell viability,percentage of G1 phases,area of calcified nodules and decrease apoptosis rate,ROS content,expression of PCNA,activity of iNOS.Besides,mRNA levels of HIF-1α and BCL-2 were enhanced and that of Caspase-3 was inhibited.Conclusion:Genistein can protect osteoblasts from hypoxia and enhance osteogenic differentiation significantly.
Keywords:HIF-1α  Genistein  Osteoblast  Cellular cycle  Apoptosis  PCNA  ROS
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