VEGF及其受体的表达对鼻内翻性乳头状瘤恶变倾向预测的意义

目的:探讨血管内皮生长因子(VEGF)及其胎肝激酶受体(flk-1)在鼻内翻性乳头状瘤(NIP)恶变前不同阶段中的表达情况。方法:应用免疫组织化学LSAB法检测NIP、非典型增生的不同阶段及恶变组织中VEGF及其受体flk-1的表达情况。应用图像分析仪对染色结果进行定量分析。结果:VEGF、flk-1阳性染色随着不典型增生程度的加重,其在上皮组织和间质结缔组织中的表达强度和范围也增加。轻度不典型增生与NIP比较,中-重度不典型增生与轻度不典型增生比较,VEGF、flk-1表达差异均有统计学意义(均P<0.01)。中-重度不典型增生组织的表达强度与鳞状细胞癌组织接近。结论:VEGF参与了NIP的病理过程,VEGF及其受体flk-1的表达随肿瘤异型程度的增加其表达强度增加,其表达水平可以作为衡量NIP恶变倾向的指标之一。

Significance and expression of VEGF and its receptor in nasal inverted papillomas and the correlation with malignant phenotype

Objective:To determine the expression of vascular endothelial growth factor(VEGF) and their receptor in nasal inverted papillomas(NIP) and to clarify the function of VEGF in the occurrence of NIPs and the correlation with malignant phenotype.Method:VEGF and its receptor(flk-1),expression were examined by immunohistochemistry using LSAB method in sections of NIP from 48 patients and squamous carcinoma from 8 patients.Result:All the epithelium together with the adjacent vascular and stroma,expressed increased positive staining of VEGF and flk-1 with the degree of atypical hyperplasia in epithelium.The VEGF/flk-1 expression in epithelium was significantly stronger in severe atypical hyperplasia than that in mild atypical hyperplasia,and same in mild atypical hyperplasia than in NIPs(P<0.01).Conclusion: VEGF/flk-1 participate in the growth of NIPs.The enhanced VEGF/flk-1 in the epithelium may be identified as one of the parameters in judging malignant transformation of NIPs.