石南藤化学成分研究

目的:对石南藤的化学成分进行研究。方法:利用正相硅胶、反相硅胶和葡聚糖凝胶Sephadex LH-20等多种柱色谱技术对石南藤的化学成分进行分离,根据核磁共振光谱等数据进行结构鉴定,对分离得到的化合物进行DPPH自由基清除活性和乙酰胆碱酯酶抑制活性检测。结果:从石南藤乙酸乙酯相分离鉴定了10个化合物,分别为:3,4-亚甲二氧基-苯甲酸(1)、香草酸(2)、苯甲酸(3)、N-p-香豆酰酪胺(4)、风藤酮(5)、风藤奎醇(6)、异风藤奎醇A(7)4、-羟基-3,5-二甲氧基-苯甲酸(8)、风藤酰胺(9)和二氢荜茇明宁碱(10)。结论:化合物1～6为首次从石南藤中分离得到,化合物28、具有一定的自由基清除活性,其EC50值分别为224.33μg/mL和11.44μg/mL,化合物1～10均无乙酰胆碱酯酶抑制活性。

Study on the bioactive constituents of Piper wallichii

Objective: To investigate the bioactive constituents in the stem of Piper wallichii.Methods: Compounds were separated by column chromatography of silica gel,ODS-A and Sephadex LH-20.Their structures were elucidated based on spectral analysis.DPPH scavenging activity and AchE inhibitory activity were tested.Results: 10 compounds were isolated and their structures were identified as 3,4-methylenedioxy-benzoic acid(1),vanillic acid(2),benzoic acid(3),N-p-coumaroyltyramine(4),futoenone(5),futoquinol(6),isofutoquinol A(7),4-hydroxy-3,5-dimethoxy-benzoic acid(8),futoamide(9),dihydropiperlonguminine(10).Conclusion: Compounds 1-6 are isolated from P.wallichii for the first time.Vanillic acid(2)and 4-hydroxy-3,5-dimethoxy-benzoic acid(8)show scavenging activity against DPPH radical with ED50 at 224.33μg/mL and 11.44μg/mL,respectively.No compound shows inhibition activity against AchE.