| 蒙药草乌芽中药效组分的电喷雾串联质谱分析 |
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| 引用本文： | 图 雅,张贵君,刘志强,王淑敏.蒙药草乌芽中药效组分的电喷雾串联质谱分析[J].医学教育探索,2009,40(5):694-697. |
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| 作者姓名： | 图 雅 张贵君 刘志强 王淑敏 |
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| 作者单位： | 1.北京中医药大学中药学院，北京 100102； 2.内蒙古民族大学蒙医药学院，内蒙古 通辽 028000； 3.中国科学院 长春应用化学研究所 新药研究室，吉林 长春 130032； 4.长春中医药大学，吉林 长春 130117 |
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| 基金项目： | 国家教育部科学技术研究重点项目(00167)；国家自然科学基金地区科学基金项目:(30860391) |
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| 摘 要： | 目的 分析蒙药草乌芽的药效组分。方法 利用电喷雾串联质谱技术对蒙药草乌芽的乙醇提取液进行了直接分析鉴定。结果 ESI-MS可以给出相对分子质量信息，ESI-MSn方法则可以从复杂体系中获得结构信息，在草乌芽中发现乌头碱、中乌头碱、次乌头碱及它们的水解产物和脂类生物碱等15种二萜类生物碱。结论 草乌芽中的药效组分二萜类生物碱为首次报道，这些生物碱的结构相似，在电喷雾串联质谱中碎裂方式相同，因此根据电喷雾串联质谱结果确定了这些生物碱的结构。
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| 关 键 词： | 草乌芽 电喷雾串联质谱 二萜类生物碱 |
Analysis on active components alignment in dried seedling of Aconitum kusnezoffii by ESI-MSn |
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| TU Y,ZHANG Gui-jun,LIU Zhi-qiang,WANG Shu-min.Analysis on active components alignment in dried seedling of Aconitum kusnezoffii by ESI-MSn[J].Researches in Medical Education,2009,40(5):694-697. |
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| Authors: | TU Y ZHANG Gui-jun LIU Zhi-qiang WANG Shu-min |
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| Institution: | 1. School of Chinese Materia Medica, Beijing University of Traditional Chinese Medicine, Beijing 100102, China; 2. College of Mongolian Medicine, Inner Mongolia Universty for Nationalities, Tongliao 028000, China; 3.Department of New Drug Research, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130032, China; 4. Changchun University of Traditional Chinese Medicine, Changchun 130117, China |
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| Abstract: | Objective To analyze the active components alignment in the dried seedling of Aconitum kusnezoffii. Methods The ethanol extract in the dried seedling of A. kusnezoffii was analyzed by the method of ESI-MSn. Results The information of molecular weight and structure could be obtained by using ESI-MS and ESI-MSn, respectively, and 15 kinds of diterpenoid alkaloids including aconitine (AC), mesaconitine (MA), hypaconitine (HA), their hydrolysis products, and lipo-alkaloids were simultaneously determined. Conclusion The diterpenoid alkaloids in the dried seedling of A. kusnezoffii are reported for the first time as its bioactive components. These alkaloids structures are identified by ESI-MSn because their structures and the fragmentation regularity are similar. |
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| Keywords: | the dried seedling of Aconitum kusnezoffii Reichb ESI-MSn diterpenoid alkaloids |
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