| HPLC同时测定麦冬中3种高异黄酮含量 |
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| 引用本文： | 曾品涛,周慧,郑一敏,胥秀英,傅善权. HPLC同时测定麦冬中3种高异黄酮含量[J]. 中国中药杂志, 2012, 37(1): 71-74 |
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| 作者姓名： | 曾品涛 周慧 郑一敏 胥秀英 傅善权 |
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| 作者单位： | 重庆理工大学药学与生物工程学院,重庆,400054 |
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| 基金项目： | 国家"重大新药创制"科技重大专项(2010ZX09401-306-2-23);重庆市科委产业化项目(CSTC,2008AB5116) |
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| 摘 要： | 采用反相高效液相色谱法同时测定麦冬中6-甲酰基沿阶草酮甲、甲基沿阶草酮甲和沿阶草酮甲的含量,选用乙腈-0.5％磷酸溶液(58:42)作为流动相,检测波长为296nm(0～14min),275nm(14～22min).6-甲酰基沿阶草酮甲、甲基沿阶草酮甲和沿阶草酮甲检测的线性范围分别为0.165～0.990(r=0.9999),0.153～0.918(r=0.9999),0.270～1.620μg(r=0.9999),平均加样同收率分别为99.76％(RSD0.95％),99.86％(RSD0.82％),99.41％(RSD1.1％).研究所建立的反相高效液相色谱法法简便快速、准确度高,可用于麦冬药材的质量控制.
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| 关 键 词： | 麦冬 高效液相色谱 6-甲酰基沿阶草酮甲 甲基沿阶草酮甲 沿阶草酮甲 含量测定 |
| 收稿时间： | 2011-04-11 |
Simultaneous determination of three homoisoflavonoids in Ophiopogon japonicus by HPLC |
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| ZENG Pintao,ZHOU Hui,ZHENG Yimin,XU Xiuying and FU Shanquan. Simultaneous determination of three homoisoflavonoids in Ophiopogon japonicus by HPLC[J]. China Journal of Chinese Materia Medica, 2012, 37(1): 71-74 |
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| Authors: | ZENG Pintao ZHOU Hui ZHENG Yimin XU Xiuying FU Shanquan |
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| Affiliation: | School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing 400050, China;School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing 400050, China;School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing 400050, China;School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing 400050, China;School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing 400050, China |
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| Abstract: | Three homoisoflavonoids, 6-aldehydo-3-ophiopogonanone A, methyl ophiopogonanone A and ophiopogonanone A from Ophiopogon japonicus were analyzed simultaneously by HPLC with acetonitrile-water containing 0.5% H3 PO4 (58:42) as the mobile phase, and the detection wavelength was set at 296 nm (0-14 min) and 275 nm (14-22 min). The mean recoveries of three homoisoflavonoids were 99.41%-99.86% (RSD 0.82%-1.05%). The linear response ranges of. 6-aldehydo-3-ophiopogonanone A, methyl ophiopogonanone A and ophiopogonanone A were 0.165-0.990 microg (r = 0.999 9), 0.153-0.918 microg (r = 0.999 9), and 0.270-1.620 microg (r = 0.999 9), respectively. This method was certified to be accurate and reliable and can be used for quality control of O. japonicus. |
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| Keywords: | Ophiopogonis Radix 6-ldehydo-3-ophiopogonanone A methyl ophiopogonanone A ophiopogonanone A HPLC quantitative determination |
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