慢性HBV感染者外周血单个核细胞分泌干扰素α功能初探

目的：旨在检测慢性HBV感染者外周血单个核细胞（PBMC）分泌干扰素α（IFN-α）的情况，以探究其外周血浆细胞样树突状细胞（pDC）和单核细胞功能是否存在缺陷。方法前瞻性纳入2012年7月至2013年3月北京协和医院肝炎门诊就诊的慢性乙型肝炎（CHB）患者16例，同期选取与之年龄相匹配的HBV携带者16例和健康人18例。采集血样，分离PBMC，分别与含未甲基化的胞嘧啶鸟嘌呤二核苷酸序列的寡脱氧核苷酸2216（CPG ODN2216）、聚肌苷酸胞苷酸（poly I：C）刺激共培养，并用酶联免疫吸附试验（ELISA）测量其分泌IFN-α量。结果经ODN2216刺激后，CHB组患者PBMC分泌IFN-α量平均为31.20（7.33～44.04）pg/ml，HBV携带者组PBMC分泌IFN-α量平均为109.91（13.74～240.27）pg/ml，健康对照组PBMC分泌IFN-α量平均为107.95（48.59～227.33）。CHB患者组显著低于健康对照组，差异具有统计学意义（Z=-2.691，P=0.007）。CHB患者组显著低于HBV携带者组，差异具有统计学意义（Z=-2.206，P=0.027）。经poly（I：C）刺激后，三组PBMC分泌IFN-α量差异无统计学意义（F=0.628，P=0.427）。结论较HBV携带者和健康对照组，CHB患者体内PBMC中树突状细胞分泌IFN-α明显下降，而单核细胞分泌IFN-α量无显著性降低。

The features of peripheral blood mononuclear cells secreting interferon-alfa in patients with chronic hepatitis B virus infection

Objective To investigate the features of peripheral blood mononuclear cell （PBMC） secreting interferon-alfa （IFN-α） and evaluate the function of pDC and monocyte to produce IFN-αin patients with chronic hepatitis B virus infection. Methods The fresh blood samples were collected from 16 patients with chronic hepatitis B, 16 age matched patients with HBV carrier and 18 age matched healthy individuals as controls from July in 2012 to March in 2013 in Peking Union Medical College Hospital. PBMCs were isolated, respectively and then stimulated in vitro by CPG oligodeoxynucleotides （CPG ODN）2216 or polyinosinic-polycytidylic acid （poly I：C） as stimulators and measured by enzyme linked immunosorbent assay （ELISA） for IFN-α production. Results With CPG ODN2216 stimulation, the level of IFN-αproduction was 31.20 （7.33-44.04） pg/ml in CHB group, 109.91 （13.74-240.27） pg/ml in HBV carrier group and 107.95 （48.59-227.33） pg/ml in healthy control group, respectively. Lower levels of IFN-αproduction in CHB group were observed comparing with in healthy control group （Z=-2.691,P=0.007）, and with in HBV carrier group （Z=-2.206,P=0.027）. There were no signiifcant differences between the three groups （F=0.628,P=0.427） with poly （I：C） stimulation. Conclusions There was no signiifcant difference in IFN-αproduction from PBMCs pulsed with CPG ODN 2216 between HBV carrier group and healthy control group. Impaired function of pDC secreting IFN-αwas found in the patients with CHB, while no signiifcant difference in monocyte secreting. It suggested immune suppression is present in patients with CHB.