散发性乳腺癌中DJ-1蛋白高表达与不良预后

目的 探讨DJ-1蛋白表达水平在散发性乳腺癌发生、发展及预后中的临床意义.方法 采用免疫组织化学法检测了包含199例散发性乳腺癌组织与160例乳腺良性增生组织的组织微阵列芯片中DJ-1蛋白的表达情况,同时应用酶联免疫吸附试验检测48例散发性乳腺癌及35例乳腺良性增生患者血清中DJ-1蛋白的水平,并分析组织中DJ-1蛋白表达水平与乳腺癌患者临床病理指标及生存资料的相关性.统计分析采用Pearson ×^2检验和独立样本t检验;生存分析采用log-rank检验绘制Kaplan-Meier曲线,Cox回归用于进行多变量分析.结果 DJ-1蛋白在乳腺良性增生组织及乳腺癌组织中阳性表达率分别为33.8％ （54/160）、48.7％（97/199）,在乳腺良性增生及乳腺癌患者血清中DJ-1蛋白平均水平分别为（25.86±7.28） ng/ml和（31.31±8.39） ng/ml,与乳腺良性增生相比,乳腺癌组织（×^2=8.182,P=0.004）及血清（t=3.161,P=0.002）中DJ-1蛋白的表达水平均显著增加;淋巴结转移阳性的乳腺癌组织与阴性组织相比,DJ-1表达水平显著增加（×^2=5.372,P=0.020）;随着组织学分级的增加,浸润性导管癌中DJ-1表达水平显著增高（×^2=5.244,P=0.022）;DJ-1阳性表达与患者生存期缩短显著相关（OS：×^2=5.427,P=0.020;×^2=4.606,DFS：P=0.032）.单变量Cox回归分析发现,DJ-1表达水平作为危险因素与患者的OS及DFS显著相关（OS：P=0.023; DFS：P=0.036）,但多变量Cox回归分析发现,DJ-1表达水平并非影响患者预后的独立风险因素（OS：P=0.561; DFS：P=0.342）.结论 在散发性乳腺癌中,DJ-1蛋白表达水平显著增高,其高表达与乳腺癌的侵袭转移、病程进展及不良预后相关.

High expression of DJ-1 protein related to poor prognosis in sporadic breast cancer

Objective To explore the clinical significance of DJ-1 protein expression in the development and prognosis of sporadic breast cancer.Methods DJ-1 protein expression was detected by immunohistochemistry in tissue microarray chips containing 199 cases of sporadic breast cancer tissues and 160 cases of breast benign hyperplasia tissues.Serous DJ-1 protein levels in 48 sporadic breast cancer cases and 35 breast benign hyperplasia cases were detected by enzyme linked immunosorbent assay.And the correlation was analyzed between DJ-1 protein expression and clinicopathologic parameters and survival data.The data were processed by Pearson ×^2 test and independent sample t-test; the log-rank test and Kaplan-Meier curve were used for survival analysis,Cox regression for multivariate analysis.Results The positive expression rates of DJ-1 protein were 33.8% （54/160） in breast benign hyperplasia and 48.7% （97/199） in breast cancer tissue respectively（×^2 =8.182,P =0.004）.Serous DJ-1 levels were （25.86 ± 7.28） ng/ml in breast benign hyperplasia and （31.31±8.39） ng/ml in breast cancer tissue respectively（t =3.161,P=0.002）.DJ-1 protein level was higher significantly in breast cancer patients with lymph nodes positive than in patients with lymph nodes negative （×^2 =5.372,P =0.020）.DJ-1 protein level was increased significantly with the increase of histological grade （×^2 =5.244,P =0.022）.DJ-1 positive expression was associated significantly with poor prognosis of breast cancer patients （OS：×^2 =5.427,P =0.020 ; DFS：×^2 =4.606,P =0.032）.Univariate Cox regression analysis showed that DJ-1 expression was a risk factor associated significantly with OS and DFS of the patients （OS：P =0.023; DFS：P =0.036）; but multivariate Cox regression analysis showed that DJ-1 expression was not an independent risk factor in the prognosis of breast cancer patients （OS：P=0.561; DFS：P=0.342）.Conclusion DJ-1 protein expression is increased significantly in sporadic breast cancer,which is as