硬棘软珊瑚醋酸乙酯部位的化学成分研究

目的研究硬棘软珊瑚醋酸乙酯部位的化学成分。方法采用正、反相硅胶柱色谱以及半制备高效液相色谱进行分离，并根据理化性质和波谱数据鉴定化合物的结构。结果分离得到11个化合物，其中3个环丁烯内酯类化合物，分别鉴定为（研-13-（2-羟基-3，4-二甲基-5-氧代-2，5-二氢呋喃。2-基）十三烷酸（1）、（S）-11-（2-羟基-3，4-二甲基-5-氧代-2，5-二氢呋喃-2-基）十一烷酸（2）、羟基二氢博伏内酯（3）；1个嘌呤类化合物，鉴定为1，3-二甲基黄嘌呤（4）；7个甾体类化合物，分别鉴定为孕甾-20-烯-3-酮（5）、孕甾-1，4，20-三烯-3-酮（6）、3β-羟基-孕甾-20-烯（7）、3p-羟基-孕甾-5,20-二烯（8）、20-羟基-孕甾-1,4-二烯-3-酮（9）、20-羟基-孕甾-1-烯-3-酮（10）、1713-羟基一雄甾-1-烯-3-酮（11）。结论环丁烯内酯类化合物是首次从该属软珊瑚中分离得到，其中化合物1和2为新化合物，而硬棘软珊瑚醋酸乙酯部位的化学成分主要为甾体类化合物。

Chemical constituents in ethyl acetate fraction from Scleronephthya sp.

Objective To investigate the chemical constituents in the ethyl acetate fraction from Scleronephthyasp.. MethOds The compounds were isolated by column chromatography （silica gel, including reversed phase） and semi-prepative HPLC, and their structures were identified on the basis of physicochemical properties and spectroscopic data. Results Eleven compounds were separated, three of which were butenolides, identified as （S）-13-（2-hydroxy-3,4-dimethyl-5-oxo-2,5-dihydrofuran-2-yl） tridecanoic acid （1）, （S）-11-（2-hydroxy-3,4-dimethyl-5-oxo-2,5-dihydrofuran-2-yl） undecanoic acid （2）, and hydroxydihydrobovolide （3）, one of which was purine, identified as 1,3-dimethylxanthine （4）, seven of which were pregnane steroids, identified as pregna-20-en-3-one （5）, pregna-l,4,20-trien-3-one （6）; 313-hydroxy-pregna-20-ene （7）, 3β-hydroxy-pregna-5,20-diene （8）, 20-hydroxy-pregna-l,4-dien- 3-one （9）, 20-hydroxy-pregna-l-en-3-one （10）, and 1713- hydroxy-androst-l-en-3-one （11）. Conclusion Pregnane steroids are the main chemical constituents in ethyl acetate fraction ofScleronephthya sp., and compounds 1 and 2 are new compounds, while butenolides are obtained from Scleronephthya sp. for the first time.