Accumulation of class I mutant p53 and apoptosis induced by carboplatin in a human glioma cell line |
| |
Authors: | Yasuhiro Hamada Masahiro Mizoguchi Satoshi O Suzuki Toru Iwaki |
| |
Institution: | (1) Department of Neuropathology, Neurological Institute, Faculty of Medicine, Kyushu University, 812-8582 Fukuoka, Japan |
| |
Abstract: | Following DNA damage, wild-type p53 increases and mediates the multiple cellular responses for the repair of DNA damage or
apoptosis. Inactivation of p53 by single-amino-acid substitutions contributes to the malignant phenotype and confers resistance
to therapy. Among tumor-derived p53 mutants, class I mutants still retain a native-like three-dimensional structure, whereas
class II mutants have unfolded DNA-binding domains. Sequencing analysis demonstrated that a human glioma cell line (U-373MG)
had only a class I mutant form of p53 of His273, which targets an Arg273 that contacts DNA but retains the native structure.
In this study, we investigated the metabolic alteration of the class I mutant p53 in apoptosis of U-373MG. The cell cycle
progression of U-373MG cells was affected by the addition of carboplatin, while the amount of mutant p53 also increased in
their nuclei. The treated cells underwent apoptosis 48h after exposure to 50 μg/ml carboplatin. Although the exact mechanism
of the class I mutant p53 in the process of apoptosis has not yet been clarified, the fact that accumulation of the activated
mutant p53 in the nucleus of U-373MG is concomitant with apoptosis, just as wild-type p53 does, implies that the class I mutant
p53 might retain the ability to participate in apoptosis. |
| |
Keywords: | p53 Class I mutant Apoptosis Chemotherapy Glioma |
本文献已被 SpringerLink 等数据库收录! |
|