不稳定性心绞痛患者KLOTHO基因C-1818T多态性分析

目的:探讨KLOTHO基因C-1818T多态性与中国苏皖地区汉族人群不稳定性心绞痛(unstable angina pec-toris,UAP)的可能关系。方法:应用聚合酶链反应(PCR)及基因芯片(gene microarray)方法,检测146例UAP患者和235例非冠心病者的KLOTHO基因C-1818T多态性。结果:与对照组相比:(1)UAP患者CC,CT和TT基因型以及T等位基因型频率分布差异无统计学意义(P值均>0.05);(2)年龄<60岁的UAP组TT基因型频率和女性UAP组TT基因型,T等位基因频率均明显升高(P值分别为0.017,0.049,0.026)。在调整相关危险因素并经多元Logistic回归分析后,TT基因型与女性UAP的发生存在相关性(P=0.041),而T等位基因与女性UAP的发生无明显关联(P=0.472)。结论:在中国苏皖地区汉族人群中,KLOTHO基因C-1818T多态性TT基因型与女性UAP的发病存在相关性。

Relationship between the KLOTHO gene C-1818T polymorphism and unstable angina pectoris

Objective: To investigate the possible association between the KLOTHO gene C-1818T polymorphism and unstable angina pectoris(UAP)in a Chinese Han population of Suwan region.Methods: This study was conducted with a case-control design including 146 patients with UAP and 235 control subjects who were free from coronary artery disease.The KLOTHO gene polymorphism was determined by polymerase chain reaction and gene microarray. Results: Compared with the control group,(1)The frequencies of CC,CT,TT genotype and the T allele of the C-1818T in UAP group was not significantly different;(2)The prevalence of the TT genetype was obveriously higher in younger(age<60)patients(P=0.017),the frequencies of TT genetype and T allele were also higher in female(P value was 0.049 and 0.026 respectively).Furthermore,after adjustment for conventional risk factors by multiple logistic regression analysis showed that the TT genotype was a significant risk factor for UAP in the female carrier(P=0.041),but the T allele had no association with UAP in female(P=0.472). Conclusion: The TT genetype of KLOTHO gene C-1818T polymorphism was associated with the susceptibility to UAP in the female among the Chinese Han population of Suwan region.