养血清脑颗粒对慢性脑缺血大鼠脑血流量及认知功能的影响

目的：观察养血清脑颗粒(YQG)对慢性脑缺血大鼠脑血流量及认知功能的影响。方法：Wistar大鼠双侧颈总动脉永久结扎造成慢性脑缺血模型,激光多普勒血流仪检测额叶皮质局部脑血流量；Morris水迷宫检测认知功能。结果：术后模型组大鼠脑血流量骤降,并呈慢性持续下降趋势(P＜0．05)；大鼠出现认知功能障碍,隐匿平台逃避潜伏期和游泳距离明显延长(P＜0．05,P＜0．01),空间探索实验逃避潜伏期明显延长,穿越次数明显减少(P＜0．01),平台象限游泳时间和距离均缩短(P＜0．01)。YQG 3个剂量组均能明显增加脑血流量,改善认知障碍(P＜0．05或P＜0．01)。结论：YQG能明显改善慢性脑缺血大鼠局部脑血流量,提高学习记忆能力,有随疗程的增加而作用增强的趋势。

Effects of Yangxue Qingnao granules on regional cerebral blood flow and cognitive functions in chronic cerebral ischemic rats

AIM:To investigate the effects of Yangxue Qingnao granules(YQG)on regional cerebral blood flow and cognitive functions in chronic cerebral ischemic rats.METHODS:Wistar rats were subjected to permanent bilateral common carotid arteries ligation in forming chronic cerebral ischemic models.Regional cere- bral blood flow in the frontal lobe cortex was measured by laser-Doppler flowmetry in different stages.The learn- ing and memory abilities were assessed with the Morris water maze.RESULTS:After the arterial ligation,the regional cerebral blood flow in the model group decreased sharply,and the reduction was progressively carried on (P＜0.05),as well as the cognitive impairment.The escape latency and distance of swimming were significantly longer than those in sham-operated group(P＜0.05,P＜0.01);the times of passing through the platform de- creased significantly(P＜0.01);and the time and distance of swimming in platform quadrant also decreased sig- nificantly(P＜0.01).After treatment with YQG,the regional cerebral blood flow increased and the learning and memory abilities were improved(P＜0.05 or P＜0.01).CONCLUSION:YQG has great promotive effect on the regional cerebral blood flow and cognitive functions in chronic cerebral ischemic rats.YQG in doses of 24, 12 g·kg~(-1) show improving therapeutic effect progressively dependent on the course of treatment.