南瓜蛋白对人胰腺癌细胞系PANC-1的细胞毒性及化疗增敏作用

目的：观察南瓜蛋白（CUS）对人胰腺癌细胞系PANC-1的细胞毒性和化疗增敏作用。方法：采用四甲基偶氮唑蓝（MTT）比色法，观察不同浓度（量效）的CUS及作用不同时间（时效）对胰腺癌细胞PANC-1的细胞毒性；采用 Chou Talalay 联合指数法判断CUS增加胰腺癌细胞对吉西他滨(GEM)的化疗敏感性。结果：CUS对胰腺癌PANC-1细胞具有显著的增殖抑制作用，0.3125, 0.625, 1.25, 2.5, 5.0, 10.0, 20.0, 40.0, 80.0 μg/mL的CUS作用于胰腺癌细胞24 h，其抑制率分别为9.16％,11.9％,14.4％,20.2％,32.0％,36.4％,44.1％,50.3％和58.6％，呈现明显的剂量依赖性；随着作用时间的延长,其抑制率明显增加, 24，48，72 h的半数抑制浓度（IC 50）分别为40.24,8.24，1.17 μg/mL，呈现明显的时间依赖性。两药在不同效应时各自所需浓度之间相互作用均为协同作用（CI<1）, 单吉西他滨用于胰腺癌细胞的IC 50为36.76 nmol/L, CUS与其合用时的IC 50为12.14 nmol/L，合用比单用时吉西他滨的剂量减少67.0%。结论：CUS对胰腺癌的细胞毒性作用呈明显的剂量依赖性和时间依赖性，与吉西他滨联合使用可增加其细胞毒作用，起到化疗增敏效应。

Cytotoxic and chemo-sensitivity enhancement effect of cucurmosin on pancreatic cancer cell line PANC-1 in vitro

Objective:To investigate the cytotoxic and chemo-sensitivity enhamcement effect of cucurmosin（CUS） on pancreatic cancer cell line PANC-1 in vitro.Methods:The cytotoxic effect of the different concentrations of CUS on PANC-1 cells in different time periods (24, 48 and 72 hours) was detected by means of MTT assay，and the chemo-sensitivity enhancement effect of CUS on gemcitabine(GEM) was evaluated by combination-index method of Chou and Talalay.Results:Cucurmosin significantly inhibited the proliferation of pancreatic cancer cell line PANC-1 in dosage-and time-dependent manner. After exposure to 0.3125,0.625,1.25,2.5,5.0,10.0,20.0,40.0 and 80 μg/mL of CUS, the inhibitory rate of the cell growth at 24 hours was 9.16％,11.9％,14.4％,20.2％,32.0％,36.4％,44.1％,50.3％and 58.6％respectively. The 50% inhibitory concentration (IC 50) at 24、48 and 72 h was 40.24 μg/mL,8.24 μg/mL and 1.17 μg/mL respectively. The combination of CUS and GEM at different concentrations showed synergic effect on inhibiting PNCA-1 cell growth (CI<1).The 50% inhibitory concentration (IC 50) of GEM decreased from 36.76 nmol/L to 12.14 nmol/L(less than 67.0%) when combined with CUS.Conclusions:Cucurmosin can significantly inhibit the proliferation of PANC-1 cells in dosage-and time-dependent manner in vitro,and combination with gemcitabine increases its cytotoxic action and provides chemo-sensitivity enhancement effect on pancreatic cancer cell line PANC-1.